Stem Cell Characterization Service

Reprogramming of human induced Pluripotent Stem Cells (hiPSCs) is a fundamental task of stem cell research. In order to provide reliable model cells for drug discovery and preclinical research, an extensive characterization of the generated hiPSC is absolutely essential. Our scientific team supports you with a comprehensive stem cell characterization service investigating your hiPSC lines and providing you with reliability and safety for your research project.

In addition to the hiPSC characterization, trenzyme also provides a customited Stem Cell Differentiation Service.

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Characterization of human induced

Pluripotent Stem Cells

The field of human induced pluripotent stem cell (hiPSC) technology is a promising tool for drug discovery and regenerative medicine.

Since the first generation of hiPSCs, numerous reprogramming techniques have been established. However, the in-vitro process of reprogramming is still not fully understood. Often, the cellular stress, induced by the reprogramming process causes genetic and epigenetic alterations that can change the cellular phenotype and affect the integrity of experiments using these cell lines.

It is therefore crucial to establish reliable molecular and functional assays to assess pluripotency and to exclude genetic aberrations due to the reprogramming process.

trenzyme’s routinely performed assays to validate your hiPSC lines include the characterization of cells for their expression of pluripotency markers via immunocytochemistry (ICC) and qPCR, molecular karyotyping, as well as the evaluation of their differentiation potential.

What clients say about us

trenzyme is one of our preferred service providers for cell line development and molecular biology services. The impressive level of personal customer liaison and the scientific competence are decisive factors for us to cooperate with trenzyme for many years and even to intensify in the area of demanding iPS cell line projects.

Dr. Margit Bauer & Dr. Ralf Heilker Boehringer Ingelheim Pharma GmbH & Co KG, Biberach, Germany
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iPSC Characterization

& Banking Service

If you work in the field of stem cell research and you are reprogramming hiPSC lines or start experiments using a unknown hiPSC line, our scientific stem cell experts support you with fully characterized and ready-to-use hiPSC master and working stocks. Accordingly, trenzyme serves as your time- and cost-saving extended workbench.

Our expert service includes:

  • Standardization of hiPSC culture parameters
  • Molecular and functional characterization of hiPSC lines
  • Banking of hiPSC master- and working stocks
  • Comprehensive hiPSC line datasheet
iPSC Stem Cell Characterization Process

Your benefits

from working with us

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Transparency & Communication

Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.

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Longstanding Expertise

With the extensive knowledge of our highly qualified scientific experts and with access to a huge network of partners and technologies, we complete your project successfully and efficiently.

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Optimized Performance

Since iPSC can be derived from healthy donors and disease patients, they serve as ideal systems for modelling disease phenotypes needed for drug screening and pharmaceutical development.

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High Quality Standards

As an ISO-certified company working with a LIMS based documentation, all projects are completed following highest standards.

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Stem Cell Characterization FAQs

What general quality control is performed on the iPSC lines?

All iPSC lines are subjected to a standard set of quality control assays, investigating sterility, mycoplasma, viability, and morphology.

What kind of assays will be performed to functionally characterize the iPSC lines?

In order to characterize iPSC lines for the expression of pluripotency markers, immunoflourescence staining and/or flow cytometry for key pluripotency markers OCT4, SOX2, SSEA-4, TRA-1-60, and TRA-1-81 will be performed. Furthermore, the ability to form all three germ layers will be investigated via directed differentiation towards endo-, meso-, and ectoderm (immunoflourescence staining, RT-qPCR).

What kind of karyotyping will be performed?

In order to evaluate genomic stability, chromosomal abnormalities, and copy number variants, molecular karyotyping via Illumina BeadArray will be applied.

See General FAQs
antje fuhrmann application & sales manager trenzyme
Antje Fuhrmann, PhD
Application & Sales Manager

We would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.

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