Stem Cell Characterization Service
In addition to the hiPSC characterization, trenzyme also provides a customited Stem Cell Differentiation Service.
Characterization of human induced Pluripotent Stem Cells
Since the first generation of hiPSCs, numerous reprogramming techniques have been established. However, the in-vitro process of reprogramming is still not fully understood. Often, the cellular stress, induced by the reprogramming process causes genetic and epigenetic alterations that can change the cellular phenotype and affect the integrity of experiments using these cell lines.
It is therefore crucial to establish reliable molecular and functional assays to assess pluripotency and to exclude genetic aberrations due to the reprogramming process.
trenzyme’s routinely performed assays to validate your hiPSC lines include the characterization of cells for their expression of pluripotency markers via immunocytochemistry (ICC) and qPCR, molecular karyotyping, as well as the evaluation of their differentiation potential.
iPSC Characterization & Banking Service
Our expert service includes:
- Standardization of hiPSC culture parameters
- Molecular and functional characterization of hiPSC lines
- Banking of hiPSC master- and working stocks
- Comprehensive hiPSC line datasheet
Your benefits from working with us
Stem Cell Characterization FAQs
What general quality control is performed on the iPSC lines?
All iPSC lines are subjected to a standard set of quality control assays, investigating sterility, mycoplasma, viability, and morphology.
What kind of assays will be performed to functionally characterize the iPSC lines?
In order to characterize iPSC lines for the expression of pluripotency markers, immunoflourescence staining and/or flow cytometry for key pluripotency markers OCT4, SOX2, SSEA-4, TRA-1-60, and TRA-1-81 will be performed. Furthermore, the ability to form all three germ layers will be investigated via directed differentiation towards endo-, meso-, and ectoderm (immunoflourescence staining, RT-qPCR).
What kind of karyotyping will be performed?
In order to evaluate genomic stability, chromosomal abnormalities, and copy number variants, molecular karyotyping via Illumina BeadArray will be applied.
Stefanie Traub, PhD
Scientific Coordination Advanced Cell Systems
We would be happy to provide you with support on your stem cell research project. Let us know your questions and requests, our scientific experts will reply shortly.