Stable Cell Line Development

Stable recombinant cell lines are used in a variety of research and development applications such as assay development, drug screening and functional studies. Our proprietary ExoIN technology is a powerful and high reliable platform for generating high quality customized assay cell lines of very competitive timelines. We will set up the best strategy to transfect your individual cell line for constitutive or inducible recombinant expression of your gene of interest and validate expression of your target gene. The assessment of functional expression and generation of cell clones are available according to your needs.

What clients say about us

We were astonished by trenzyme’s recently delivered custom ExoIN cell lines. Even “difficult-to-express” constructs show stable and robust expression. I highly recommend trenzyme’s cell line development service.

PD Dr. Dieter Wicher Max Planck Institute for Chemical Ecology, Jena, Germany
clients logo max planck institute for chemical ecology trenzyme

Relevant Publications

A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology. Int. J. Cosmet. Sci. 2022; 44: 216– 231. (2022)

Welzel J, Grüdl S, Banowski B, Stark H, Sättler A, and Welss T


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Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine (2021)

Goyal P, Venkatraman Pai H, Kodali P, Vats B, Vajpai N, Annegowda S, Mane K, Mohan S, Saxena S, Bangalore Veerabhadraia A, Palande M, Sasankan Nair P, Chandrashekar More D, Rao Karudumpa U, Jyothirmai K, Bhattacharya A, Almeida F, Gulab Khyade S, Gouda S, Ranayhossaini DJ, Reddy Moole P, Smith JP, Barve A, Melarkode R, and Ullanat R


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Development and validation of a cell based assay for the detection of neutralizing antibodies against recombinant insulins. J. Immunol. Methods 452, 53–62. (2018)

Chatterjee S, Vashishta L, Waichale VS, Nayak VG, Melarkode R, Donnelly CM, Vallano PT, Chirmule N, and Sengupta N


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A botulinum toxin–derived targeted secretion inhibitor downregulates the GH/IGF1 axis. J. Clin. Invest. 122, 3295–3306. (2012)

Somm E, Bonnet N, Martinez A, Marks PMH, Cadd VA, Elliott M, Toulotte A, Ferrari SL, Rizzoli R, Hüppi PS, et al.


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Toll-like receptor 4 variant D299G induces features of neoplastic progression in Caco-2 intestinal cells and is associated with advanced human colon cancer. Gastroenterology 141, 2154–2165. (2011)

Eyking A, Ey B, Rünzi M, Roig AI, Reis H, Schmid KW, Gerken G, Podolsky DK, and Cario E


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Stable Cell Line Development FAQs

Does the ExoIN technology also work for supension cell lines and adherent cell lines?

Yes, the ExoIN technology works for both adherent and suspension cell lines.

Which cell lines have you already worked with?

We already worked with dozens of human (e.g. HT1080, Jurkat,…) and non-human cell lines (e.g. NIH3T3, PC12,…).

Which methods do you use for generation of single cell clones?

Routinely we apply flow cytometry based cell sorting or limiting dilution for single cell cloning. For difficult target cell lines we also offer colony picking in soft agar.

See General FAQs
antje fuhrmann application & sales manager trenzyme
Antje Fuhrmann, PhD
Application & Sales Manager

We would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.

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