High-Yield Recombinant Protein Production

We provide protein expression services for high-yield protein production in E. coli, yeast and mammalian cells using  individually optimized fed-batch processes in our bioreactors. We deliver your target protein in the desired amount with the highest quality.


Key Features of High-Yield Protein Production

  • Highly individualized service – production scale adapted to your needs
  • Extensive process control and optimization
  • Complete upstream and downstream process development available – gene-to-protein manufacturing
  • Protein production in optimized shake flasks or bioreactors
  • State-of-the-art production and purification methods
  • Optimization of protein expression – different options available: host expression screening and protein productivity screening
  • Optimization of protein purification
  • Available also for labeled proteins
  • High reproducibility
  • Detailed documentation and quality control

“We highly appreciate the collaboration with trenzyme on account of the following reasons: trenzyme provides high quality material and services. The ‘outstanding’ team gives excellent advices, is skilled and professional. Direct and uncomplicated communication was possible at all times.”

Dr. Roland Lange

Idorsia Pharmaceuticals Ltd, Allschwil, Switzerland


Bacterial Cells

  • E.coli strains (common and proprietary expression strains available)
  • Protein production in optimized shake flask cultures
  • Protein production using computer controlled fed-batch process (bioreactor)


  • Pichia pastoris, Kluyveromyces lactis or Saccharomyces cerevisiae (different strains available)
  • Protein production in optimized shake flask cultures
  • Protein production using computer controlled fed-batch process (bioreactor)

Mammalian Cells

  • Different proprietary HEK and CHO suspension cell lines available
  • Protein production in optimized shake flask cultures (multi L scale)
  • Available for transient and stable production processes



Process of High-Yield Protein Production Service

diagram of the process of high-yield protein production

High-Yield Production FAQs

Is labeled high-yield protein production also available?

Yes, we have developed a protocol for high-yield production of labeled proteins in bioreactors at high-cell densities.

What is the typical yield and scale in your high-yield protein production?

The typical yield is not easy to predict, but can reach up to gram-quantities per liter of high-cell density fermentation runs (E.coli and Yeast). The scale can reach up to 8 l for bioreactors and 10 l for cell culture.

Are purification conditions native or denaturing?

Generally, we offer native purification conditions. Depending on target protein properties, denaturing purification can also be offered for proteins.

Reinhold Horlacher, PhD

Reinhold Horlacher, PhD

Managing Director & CSO

We would be happy to provide you with support on your protein research project. Let us know your questions and requests, our scientific experts will reply shortly.

Get In Contact With Our Scientific Experts

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