Stem Cell Differentiation Service
Human induced Pluripotent Stem Cells (hiPSCs) have the ability to multiply indefinitely and to differentiate into all cell types of the human body. Accordingly , they provide a limitless supply of hard-to-source cell types and can thus help to develop highly physiological models cells.
Our scientific team offers a profound stem cell knowledge and function as extended workbench for your routine iPSC differentiation projects. Furthermore, to streamline the pharmaceutical development process of our clients, trenzyme is developing new protocols for iPSC differentiation into cells of various lineages according to your specific project requirements.
Differentiation of human induced Pluripotent Stem Cells
hiPSCs can be routinely generated from somatic cells of healthy donors or even patient-specific disease samples. After differentiation into tissue-specific cell types like neurons, cardiomyocytes or hepatocytes, hiPSCs can serve as next generation disease models in-a-dish, facilitating many applications in basic research, drug discovery and preclinical development.
Up to now, many applications in academia, biotech and pharmaceutical industry still rely on immortalized cancer cell lines as model system with all the drawbacks like genetic instability, aberrant gene expression patterns and dysfunctional signal pathways. Even the use of human primary cells has severe disadvantages like ethical problems, donor-to-donor variability and other general limitations (e.g. availability, limited expansion).
However, hiPSCs do not exhibit these limitations, have an unlimited expansion potential and can be differentiated into all cell types of the three germ layer lineages (ectoderm, mesoderm, endoderm). Moreover, intermediate differentiation stages can be further amplified.
“trenzyme is one of our preferred service providers for cell line development and molecular biology services. The impressive level of personal customer liaison and the scientific competence are decisive factors for us to cooperate with trenzyme for many years and even to intensify in the area of demanding iPS cell line projects.”
Run-Through Differentiation Service
If you have a proven hiPS cell line and an already established differentiation protocol including validated QC markers available, we will be able to serve as your time- and cost-saving extended workbench and provide you with the differentiated cells ready-to-use for your assays.
Our expert service includes:
- Feasibility check
- Continuous reporting accompanying the whole project
- Final report at the end of the project (protocol procedures, marker analysis)
- Generated cells
Customized Differentiation Service
If you do not have an established differentiation protocol and may also need to source the hiPS cell line as starting point, our experts will be able to support you with a milestone based R&D project.
We have extensive knowledge in development of protocols or may have the required protocol readily available. With access to a huge network of partners and technologies, we can ensure the progress of such a custom R&D project generating your desired cell type.
Our highly modular expert service includes:
- State-of-the-art literature research & feasibility check
- Close collaboration & technical discussion
- Continuous reporting accompanying the whole project
- Final report at the end of the project & delivery of test samples (protocol procedures, marker analysis)
- Generated cells
Services like karyotyping, pluripotency test, tri-lineage differentiation or cell specific data packages based on transcriptomic (qPCR), proteomic (FACS, IF) or even functional level are usually part of the customized differentiation service package. On request, they are also available for the run-through differentiation service package.
Your benefits from working with us
Transparency & Communication
Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.
Longstanding Expertise
With the extensive knowledge of our highly qualified scientific experts and with access to a huge network of partners and technologies, we complete your project successfully and efficiently.
Groundbreaking Technology
Since iPSC can be derived from healthy donors and disease patients, they serve as ideal systems for modelling disease phenotypes needed for drug screening and pharmaceutical development.
High Quality Standards
As an ISO-certified company working with a LIMS based documentation, all projects are completed following highest standards.
Publications
Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96‑Transwell air–liquid interface model. Sci Rep 11, 17028 (2021) - Bluhmki, T., Traub, S., Müller, AK. et al.
Within this work an hiPSC-derived and miniaturized AT2-like cell model system has been established, which provides a unique opportunity to study IPF in vitro. The combination of progenitor cell cryopreservation, and miniaturized cell maturation including a possible robotic integration opens new routes for target-focused and phenotypic drug discovery as well as basic research. The study was conducted in collaboration between trenzyme GmbH, Boehringer Ingelheim Pharma GmbH & Co KG and the University of Konstanz.
You can read the full paper here.
Stem Cell Differentiation FAQs
How long does it take to generate an iPSC-derived cell type?
It’s depending on the cell type and the applied differentiation protocol. Together with our clients, we select a reliable differentiation protocol and define reasonable project milestones.
What kind of quality control is performed to ensure a successful differentiation?
We routinely perform immunofluorescence staining, FACs analysis and qPCR in order to characterize the stages of differentiation. For special read-outs, do not hesitate to contact our experts.
How can I get in touch during the realization of the project?
During the project, regular telephone calls, email correspondence and short reports ensure detailed exchange of information and results. Furthermore, technical discussions will help to implement key experiment layouts according to your needs.
Stefanie Traub, PhD
Scientific Coordination Advanced Cell Systems
We would be happy to provide you with support on your stem cell research project. Let us know your questions and requests, our scientific experts will reply shortly.
Get In Contact With Our Scientific Experts