Process
of Stable Cell Line Development
trenzyme’s ExoIN Technology
Modules & Explanation
The ExoIN technology links target protein expression to the expression of a resistance marker. The target, the selection marker and the ExoIN module are transcribed together as a single mRNA transcript. Subsequent efficient cotranslational cleavage at the ribosome yields the desired target protein unmodified and functional in a 1-to-1 stoichiometry with the selection marker. This means that once selected, the stable cell pool transcribes the target protein almost homogeneously (e.g. reference protein expression GFP > 90%).
This unique technology allows an easy and highly efficient selection of homogeneous cell populations – in consequence, time consuming and expensive single cell cloning is no longer necessary for many applications.
Main Advantages
of trenzyme’s ExoIN Technology
What clients say about us
We were astonished by trenzyme’s recently delivered custom ExoIN cell lines. Even “difficult-to-express” constructs show stable and robust expression. I highly recommend trenzyme’s cell line development service.
Max Planck Institute for Chemical Ecology, Jena, Germany
Broad experience
with cell lines and target classes
Cell Lines
Target Classes
Quality Control
Assays
To ensure highest quality and validity for our final products, we offer a broad range of quality control assays for our cell line development services.
Included Analysis:
Optional Analysis:
Further Options
for Cell Line Development
Antje Fuhrmann, PhD
Application & Sales ManagerWe would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.