Expression Host Screening
Recombinant expression of a new, unknown or very challenging target protein is often performed sequentially in different hosts and needs a deep expertise on various expression systems. This takes a lot of valuable time and makes costs unpredictable. Our host protein expression screening service provides a cross organism expression platform which enables parallel screening of protein expression in different expression organisms and strains to detect the best system for your individual needs.
Additional Service Options:
Fig.1: Sequential vs. parallel model – in comparison to the conventional host finding strategy the optimized expression host screening saves maximum of time and is highly efficient.
Protein Productivity Screening
Selection of the best E.coli expression system/conditions
E.coli is often chosen as the preferred expression host, as it is usually a robust and cost-effective system. However, many protein targets are expressed poorly in E. coli or are synthesized as insoluble, inactive inclusion bodies. Our protein productivity screening service offers high-throughput screening of combinations of different expression vectors, expression strains and appropriate media using a 48-well based microfermenter system (BioLector) which monitors growth, disolved oxygen (DO), pH and fluorescence in every well. This protein productivity screen allows us to identify the best expression construct, strain and conditions to express and upscale production of your target.
Fig.2: One possible set-up to test up to 48 conditions in parallel.
Additional Service Options: