SARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), lyophilized formulation
Recombinant protein of the receptor binding domain (RBD) of SARS-CoV-2 (COVID-19) Spike S1 from Wuhan pneumonia virus with C-terminal His-Tag, lyophilized formulation.
- 100µg – 300€
- more on request – bulk and gram quantities available
Shipped next working day
|Product Name||SARS-CoV-2 (COVID-19) Spike S1 Protein (RBD), lyophilized formulation|
|RefSeq Links||NC_045512.2; MN908947.3; YP_009724390.1; QHD43416.1; GeneID: 43740568|
|Synonyms||SARS-CoV-2; coronavirus; SARS-CoV-2 spike RBD; SARS-CoV-2 spike protein; 2019-nCoV; COVID-2019; COVID-19|
|Species||SARS-CoV-2; Wuhan seafood market pneumonia virus|
|Sequence without tags (AA 319-541)||RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYA
|Expression Host||human, HEK293|
|Formulation||PBS, pH 7,4|
|Format||Lyophilized and shipped at room temperature|
|Purity||> 85% as determined by SDS-PAGE|
|The spike (S) glycoprotein of coronaviruses is essential for binding of the virus to the host cell at the beginning of the infection process. The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein is responsible for membrane fusion and is therefore required for virus entry and cell fusion. The target protein is also a major immunogen and a possible target for entry inhibitors.
The SARS-CoV-2 spike (S) protein is a large type I transmembrane protein composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain (RBD) responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2). The S2 subunit mediates fusion between the viral and host cell membranes. The S1 RBD protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
SARS-CoV-2 Spike S1 (RBD) recognizes hACE2 (ECD) with an affinity constant of 1 nM as verified by biolayer interferometry
SARS-CoV-2 Spike S1 (RBD) is highly pure and forms stable monomers of 28 kDa as verified by SEC
Biolayer interferometry binding analysis (green lines) of hACE2 (ECD, processed), tag-free to immobilized SARS-CoV-2 Spike S1 (RBD), His-tag on Ni-NTA Dip and Read™ Biosensors. Grey lines correspond to a global fit of the data using a 1:1 binding model.
Size exclusion chromatography (SEC) of purified SARS-CoV-2 Spike S1 (RBD), His-tag protein, determination of elution profile by absorbance at 280 nm (blue line).
Analysis of released N-glycans by HILIC (example data). More and lot specific analytical data available on request (provided by our partner Biofidus AG).
Further recombinant SARS-CoV-2 proteins
Reinhold Horlacher, PhD
Managing Director & CSO
We would be happy to provide you with your desired protein and to support you on your protein research project. If you have any questions or requests, our scientific experts will be happy to answer them shortly.
Get In Contact With Our Scientific Experts For Your Executable Quote Or Your Bulk Order