Recombinant Protein plus Polyclonal Antibody – Service Package

Feasibility Check

Quote and Project Plan

• Once the individual project requirements have been discussed, our scientific experts will conduct an initial feasibility check with the protein sequence (e.g. literature check, database check, product check, etc.) in order to define the optimal service strategy for this specific target.
• The detailed quote for the project will be sent to you via email or by secure data transfer of your choosing.
• After the order has been placed and confirmed, the project will start within one working day. From this point onwards, the project will be managed by our dedicated project management team, who will keep you informed at all times to ensure the project is completed successfully.

Template DNA

• Template DNA is provided by the customer: The plasmid DNA contains the codon-optimized DNA of the protein of interest (POI).
• An alternative solution is the Gene Synthesis Service by trenzyme (available as an add-on). This service offers gene synthesis of codon and DNA structure optimized DNA.

Plasmid Generation & Cloning

Small Scale Pilot Study

To maximize efficiency and save the customer significant time and money, we carry out a small-scale pilot study. These preliminary assessments help us to determine in advance whether the target protein can be expressed in E.coli and whether the purification process is feasible.

Validation of soluble or insoluble target protein expression in E. coli and feasibility of purification prior to production:

• Transformation of construct into expression strain
• Generation of cryostock
• Small-scale protein expression in shake flask culture
• Small-scale purification by mechanical cell lysis and affinity chromatography from soluble fraction of culture pellet (batch mode - insoluble targets will be purified under denaturing conditions during scaled up production)
• Validation of successful target protein purification by SDS-PAGE and Western blot
• Compilation of documentation of pilot study
• Guarantee: After a successfully completed pilot study, the outcome of this service package (purity and yield) is guaranteed
• Flexibility and Efficiency: If expression and purification is not feasible as desired, the project can be stopped at this point. No further costs or time are incurred. However, we won't leave you alone with your challenging protein target - our custom protein production service offers a variety of highly customized solutions to achieve the project goal under modified and optimized conditions.

Production: Protein Expression

Production: Protein Purification (soluble or insoluble)

• One-step purification of the target protein at 1 ml resin scale (from soluble or insoluble fraction, depending on the solubility of the target protein as identified during the pilot study - insoluble targets will be purified and delivered under denaturing conditions in a special buffer suitable for immunization)
• Buffer exchange into storage buffer suitable for immunization
• Concentration by ultrafiltration

Final guaranteed yield: up to 3 mg (2 mg will be used for immunization, up to 1 mg will be available for your experiments) and guaranteed purity: 90%

Final Quality Control of Protein

• Analysis by SDS-PAGE and Western Blot
• Assessment of final concentration and purity
• Compilation of comprehensive datasheet

In case that we are able to produce more than the guaranteed final amount of target protein, this excess protein can be purchased.

Immunization

• Immunization of two rabbits using up to 2 mg of the purified protein
• Collection of pre-immune sera and selection of suitable animals
• Collection of immune sera

The standard immunization schedule will take 49 days.

Final QC of Antiserum by ELISA

The titer of the antisera from day 28 and 49 will be determined by ELISA

Shipment of Protein plus Antibody

• Global shipping with minimal delivery times
• Our logistics team and trusted partners guarantee that all products reach you in excellent condition and without any compromise in quality
• Collaboration with reputable international carriers operating an extensive logistics network
• Full shipment transparency through continuous parcel tracking — your tracking number will be provided upon dispatch
• Proteins and cell lines are typically shipped on dry ice to maintain product stability. Alternative shipping options, such as dry shippers, blue ice, or ambient conditions, can be arranged upon request
• We ensure careful handling and responsible transport of all products, as environmental sustainability and climate protection are key elements of our corporate philosophy (see ➥ Code of Conduct)

Deliverables

Alongside your cryopreserved cells, you will receive extensive documentation comprising:

• A data sheet specifying the precise culture conditions and medium formulations
• Records detailing the growth behavior of the cells
• Optional: a full report covering the complete adaptation workflow

Timeline

The temporal course is principally determined by the specific cell line, the cellular doubling time, and the nutrient composition of the medium to which the cells are adapted (low versus high nutrient content). Accordingly, the adaptation timeline typically spans approximately 6 to 12 weeks.