Recombinant Protein plus Polyclonal Antibody – Service Package

A fixed price package for generation of a monoclonal antibody does not exist, since these types of services are individually designed in collaboration with our partner for immunization according to your exact needs.

Yes, we also guarantee the quality of an antiserum generated (polyclonal antibody). The final titer will be tested by ELISA.

No, generally we do not add preservatives to the proteins since they may influence the protein activity. But if you have a preferred storage buffer, we can use your buffer.

To generate a polyclonal antibody, an antigen (recombinant protein) is injected into a host animal to trigger an immune response. After multiple booster injections, blood is collected, and the serum containing a diverse mix of antibodies is separated. The antibodies, which recognize different epitopes of the antigen, can be further purified using methods like affinity chromatography. This results in a polyclonal antibody preparation suitable for applications like ELISA, Western blot, or immunostaining. The advantage of polyclonal antibodies is their high sensitivity due to recognition of multiple epitopes on the target protein.

The quality of an antibody is highly dependent on the quality of the antigen used for immunization. Key factors influencing antigen quality include purity, proper folding, correct epitope conformation, post-translational modifications, buffer composition, and excipients. To ensure optimal antibody production, it is crucial to start with a high-quality antigen. trenzyme offers a comprehensive service that provides high-quality antigens, enabling the seamless production of high-quality antibodies in a single package.