Recombinant Protein Expression in Mammalian Cells

Our comprehensive service package includes cloning, recombinant protein expression in mammalian cells (e.g. HEK or CHO cells) and high-quality recombinant protein purification of your secreted target protein. Rely on our many years of experience in recombinant protein expression in mammalian cells – we look forward to assisting you with your project.

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Recombinant Protein Expression Service in Mammalian Cells – Service Package for Standard Protein Targets

For standard proteins, this is the service package of choice if you require limited quantities of recombinant protein expressed in mammalian cells for example for initial target characterisation or early stage production. Furthermore, the manufacturability and production data obtained from this mammalian service package can serve as a valuable starting point for future upscaled and/or refined production of your target protein.

If you have a challenging target, need a larger quantity or have any other project requirements, we are happy to fulfill them with our ➥highly customized protein production services.

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Process of Recombinant Protein Production in Mammalian Cells

Recombinant-Protein-Expression-Service-in-Mammalian-Cells_Process-Steps_trenzyme-GmbH

trenzyme's Protein Service includes the following points. Open Hand with vial.

Protein Expression in Mammalian Cells – Service Package Includes

All steps from cloning to purification
Final yield: starting at 1 mg purified protein (more on request)
Final purity: > 90 %
Short processing time: 4 business weeks (for standard proteins) including cloning, expression, purification and QC
As starting material we can either use an existing expression vector construct from the client or start with codon-optimized gene synthesis (additional service option)
Small scale pilot study for evaluation of manufacturability – not needed for known targets
Delivery of test samples from pilot study if desired
Use of optimized and reliable mammalian expression systems
Comprehensive datasheet

Additional service options:

➥Gene synthesis and ➥High-Throughput Protein Expression Screening as starting point
Purchase of additionally produced protein
Extended Quality Control: e.g. mass spectrometry, endotoxin detection, Western Blot or ELISA
Low endotoxin purification and endotoxin removal
For larger protein quantities, more challenging targets or more specific project requirements, ➥highly customized protein production services are available

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trenzyme’s Mammalian Recombinant Protein Expression Service – Your Benefits

Guarantee – Pay Only When We Succeed!

Following a feasibility analysis and successful pilot study, we guarantee the final quantity and purity of your target protein within the specified timeframe. You only pay the production run if we succeed!

Comprehensive Service Package

A comprehensive package of services for the expression and purification of recombinant proteins at a fixed price and on a fixed scale.

Transparency & High Quality

Our dedicated project management team will guide your project through every step of the process and will keep you constantly up to date with progress. We are committed to transparency and high quality service.

Free Feasibility Analysis & Consulting

Our free feasibility check of your protein sequence is the basis for our free individual consultation to define the ideal service strategy for your target leading to success.

What Our Clients Say About Us

“(…) For any future project we will preferably entrust in trenzyme’s protein production services.”

Dr. T. Hettmann, Probiodrug AG

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Standard Protein or Demanding Target Protein – Free Feasibility Check and Pilot Study

During an initial free one-to-one consultation, our scientific experts will perform a feasibility check (e.g. literature check, database check, product check, etc.) on your protein sequence to define the ideal service strategy for your specific target.

To maximize efficiency and save you significant time and money, this service includes a small-scale pilot study. This is a preliminary assessment of whether the target protein can be expressed in mammalian cells and whether the purification process is feasible. Test samples to check the functionality and quality of the purified target protein in your hands are available on request.

The big advantage for you: After pilot-scale evaluation, we guarantee final yield, purity and timeline – you only pay for production if we succeed!

If your target sequence is identified as a feasible protein by our initial screening tools, we offer the standard recombinant protein expression in mammalian cells service package with guarantee.
If your target sequence has been identified as a challenging protein or you require high yield production (e.g. > 10 mg), we offer highly modular, customized solutions with or Custom Protein Production Service.

Protein Expression and Purification Service_Decision Path

Recombinant Protein Production in Mammalian Cell Expression System FAQs

My target protein should be glycosylated - which expression host do you recommend?

For generation of glycosylated proteins we offer different expression systems like Yeasts, insect cells (Baculo) or also mammalian cell lines. Depending on the nature of your target protein and your exact needs, we will propose the most suitable expression host and system for your project.

What starting materials are required to begin a project?

We only need the amino acid sequence or uniprot reference number of your desired protein.

Why does my protein show a higher (or lower) MW on SDS-PAGE or Western blot as calculated?

Posttranslational modifications like glycosylation or very rigid protein structures may have influences on protein properties and running behaviour. Sometimes proteins run higher, seldom lower and sometimes they run as a smear.

See General FAQs

reinhold-horlacher-managing-director-trenzyme

Reinhold Horlacher, PhD

Managing Director & CSO

We would be happy to provide you with support on your protein research project.

Get In Contact With Our Scientific Experts

Design Your Protein Now!

Use our user-friendly protein configurator to create your individual protein according to your needs. Click on the button below to get started!

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Questions or Individual Quote?

Let us know your questions and requests by simply filling out the following contact form. Our scientific experts will get in touch with you immediately to discuss your request.

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