Recombinant Protein Expression in Mammalian Cells – Service Package

Our comprehensive service package includes cloning, recombinant protein expression in mammalian cells (e.g. HEK or CHO cells) and high-quality recombinant protein purification of your secreted target protein. Rely on our many years of experience in recombinant protein expression in mammalian cells – we look forward to assisting you with your project.

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Service for Recombinant Protein Expression in Mammalian Cells_trenzyme GmbH

Recombinant Protein Expression Service in Mammalian Cells

Service Package

For standard proteins, this is the service package of choice if you require limited quantities of recombinant protein expressed in mammalian cells for example for initial target characterisation or early stage production. Furthermore, the manufacturability and production data obtained from this service package can serve as a valuable starting point for future upscaled and/or refined production of your target protein.

If you have a challenging target, need a larger quantity or have any other project requirements, we are happy to fulfill them with our highly customized protein production services.

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Process of

Recombinant Protein Expression in Mammalian Cells


Protein Expression in Mammalian Cells – Service Package Includes

  • All steps from cloning to purification
  • Final yield: starting at 1 mg purified protein (more on request)
  • Final purity: > 90 %
  • Short processing time: 4 business weeks (for standard proteins) including cloning, expression, purification and QC
  • As starting material we can either use an existing expression vector construct from the client or start with codon-optimized gene synthesis (additional service option).
  • Small scale pilot study for evaluation of manufacturability – not needed for known targets
  • Delivery of test samples from pilot study if desired
  • Comprehensive datasheet

Additional service options:

  • Gene synthesis as starting point
  • Purchase of additionally produced protein
  • Extended Quality Control: e.g. mass spectrometry, endotoxin detection, Western Blot or ELISA
  • Low endotoxin purification and endotoxin removal (Kommt das weg?)

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Your Benefits

From Working With Us

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Guarantee – Pay Only When We Succeed!

Following a feasibility analysis and successful pilot study, we guarantee the final quantity and purity of your target protein within the specified timeframe. You only pay the production run if we succeed!

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Comprehensive Service Package

A comprehensive package of services for the expression and purification of recombinant proteins at a fixed price and on a fixed scale.

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Transparency & High Quality

Our dedicated project management team will guide your project through every step of the process and will keep you constantly up to date with progress. We are committed to transparency and high quality service.

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Free Feasibility Analysis & Consulting

Our free feasibility check of your protein sequence is the basis for our free individual consultation to define the ideal service strategy for your target leading to success.

What Our Clients Say About Us

We highly valued the fast and excellent communication and the high flexibility of the team! For any future project we will preferably entrust in trenzyme’s protein production services.

Dr. Thore Hettmann Probiodrug AG, Halle/Saale, Germany
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Free Feasibility Check and Pilot Study

During an initial free one-to-one consultation, our scientific experts will perform a feasibility check (e.g. literature check, database check, product check, etc.) on your protein sequence to define the ideal service strategy for your specific target.

To maximize efficiency and save you significant time and money, this service includes a small-scale pilot study. This is a preliminary assessment of whether the target protein can be expressed in E.coli and whether the purification process is feasible. Test samples to check the functionality and quality of the purified target protein in your hands are available on request.

The big advantage for you: After pilot-scale evaluation, we guarantee final yield, purity and timeline – you only pay for production if we succeed!

  • If your target sequence is identified as a feasible protein by our initial screening tools, we offer the standard recombinant protein expression in mammalian cells service package with guarantee.
  • If your target sequence has been identified as a challenging protein or you require high yield production (e.g. > 10 mg), we offer highly modular, customized solutions with or Custom Protein Production Service.
Protein Expression and Purification Service_Decision Path
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Recombinant Protein Expression in Mammalian Cells FAQs

My target protein should be glycosylated - which expression host do you recommend?

For generation of glycosylated proteins we offer different expression systems like Yeasts, insect cells (Baculo) or also mammalian cell lines. Depending on the nature of your target protein and your exact needs, we will propose the most suitable expression host and system for your project.

What starting materials are required to begin a project?

We only need the amino acid sequence or uniprot reference number of your desired protein.

Why does my protein show a higher (or lower) MW on SDS-PAGE or Western blot as calculated?

Posttranslational modifications like glycosylation or very rigid protein structures may have influences on protein properties and running behaviour. Sometimes proteins run higher, seldom lower and sometimes they run as a smear.

See General FAQs
Reinhold Horlacher CEO trenzyme
Reinhold Horlacher, PhD
Managing Director & CSO

We would be happy to provide you with support on your protein research project. Let us know your questions and requests, our scientific experts will reply shortly.

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