Recombinant Protein Expression Service in Insect Cells
Highly Customized Service
The Insect Protein Expression System utilizes insect cells (such as Sf9 or Sf21) to produce recombinant proteins. It is particularly useful for proteins that require post-translational modifications, proper folding, or are challenging to express in bacterial systems. Compared to bacterial expression, insect cells offer a eukaryotic environment, enabling glycosylation, phosphorylation, and disulfide bond formation. This system is widely used for producing enzymes, membrane proteins, and vaccine candidates with high yield and functionality.
Our experienced protein experts offer a highly customized protein expression service in insect cells to provide the ideal and bespoke solution for your demanding target by delivering results at high speed. Transient transfection (baculovirus-free system) in insect cells is a rapid method of protein expression that significantly reduces the time from gene to protein by eliminating the time-consuming step of virus generation. This also allows highly parallel screening of different expression constructs and target variants in days rather than weeks.
trenzyme’s insect cell expression system is optimized for the expression of complex proteins that require post-translational modifications such as glycosylation or phosphorylation to ensure correct folding and functionality. By using the unique capabilities of our baculovirus-free insect cell expression systems, we provide a versatile, ultra-fast and efficient platform for producing proteins that meet the exact requirements of scientific research and pharmaceutical development.
Process of
Recombinant Protein Expression Service in Insect Cells (Baculovirus-Free)
Recombinant Protein Expression Service in Insect Cells (Baculovirus-Free)
Your Benefits
Recombinant Protein Expression Service in Insect Cells
Service Includes
Recombinant Protein Expression Service in Insect Cells
Classical Baculovirus Expression vs. Baculovirus-Free Expression System

Baculovirus-Free Protein Expression in Insect Cells
- Rapid target expression (approx. one week). Plasmid-based systems are often faster, as no virus production and amplification is necessary.
- The use of plasmids is often more cost-effective, as the production and maintenance of virus stocks can be dispensed with.
- Production is more consistent as there are no mutations or variability due to viral amplification cycles.
Baculovirus-Free Protein Expression in Insect Cells
- Large quantities of DNA are required for transfection
- Depending on target, yield may be lower than with baculovirus-mediated expression
Baculovirus-Based Protein Expression in Insect Cells
- More suitable for repeated large-scale protein production projects.
- Baculovirus systems enable expression of of complex or large multisubunit proteins.
Baculovirus-Based Protein Expression in Insect Cells
- The production of baculoviruses is very time-consuming due to multi-step virus creation and is therefore not really practical for one-off production.
- Baculoviruses cannot be amplified indefinitely, they lose quality over time. Plasmids are always of the same quality, and mutations can be detected immediately by sequencing.
- Lysis of host cells after infection with baculoviruses (possible negative influence on protein quality).