High-Throughput Protein Expression Optimization Service
Maximize the Final Yield, Activity and Quality of Your Target Protein
Do you have a target protein that is poorly expressed? Our highly customized high-throughput protein expression optimization service is designed to maximize the productivity, purity, solubility, stability and biological activity of your difficult-to-express target protein. trenzyme’s dedicated protein experts provide the fastest way to identify the optimal expression strategy for your difficult-to-express target protein through parallel high-throughput screening of different expression strains and cell lines and culture conditions such as growth media and additives, temperatures, induction conditions, extraction methods and other parameters.
With our high-throughput protein expression optimization platform, we are able to test over 1.000 conditions to identify the ideal conditions for expressing your protein. Depending on the existing knowledge of your target protein, your needs and the goal of the project, we can offer you different protein expression screening variants to overcome existing limitations and challenges and to take your innovation to the next level.
High-Throughput Protein Expression Optimization Service
Screening for the Ideal Expression Strains and Culture Conditions for Your Target Protein
Fig.1: One possible set-up to test up to 48 conditions in parallel. * The best expression construct has been selected by our high-throughput protein expression screening service.
Once the best expression construct/target variant and expression host have been selected (➥ high-throughput protein expression screening service), our high-throughput protein expression optimization service can increase the final yield and quality of your protein by further optimizing expression strains and culture conditions such as growth media and additives, temperatures, induction conditions, extraction methods and other parameters. This streamlined process development is also done in small scale and high throughput to reduce timelines and costs and maximize the output. We use a modern microbioreactor platform to perform up to 48 fermentations simultaneously in a single microtiter plate with online monitoring of valuable process data like biomass, DO, pH and fluorescence. The proteins produced are then screened for yield and quality. This can be done using various methods such as SDS-PAGE, Western Blot, ELISA or enzymatic activity. The results are analysed to identify the optimal conditions for protein expression. Combined with purification data also in a high-throughput format, the process is well prepared for upscaling and production of large quantities of your target protein.
High-Throughput Protein Expression Optimization Service
List of Parameters for Protein Expression Optimization
Strains and Cell Lines
E.coli
Mammalian
Yeast
Insect Cells
Culture Conditions
Temperature
Growth Media & Additives
Induction Conditions
Extraction Methods
Further Parameters
High-Throughput Protein Expression Optimization Service
Main Adavantages
High-Throughput Miniaturized Screening
We screen several key process parameters such as expression strains, temperature, growth media composition, induction time and even construct small-scale variants in a 48-well microbioreactor system with full documentation of key process parameters such as DO, pH, cell density and fluorescence.
High Standards of Quality
Our dedicated scientific project management team will guide your project through the entire process and provide you with regular updates on the current status of the project. This allows for high flexibility, efficiency and maximized project success. Our top priorities are high quality standards, close collaboration with our clients and total transparency.
Upscaling Made Easy
Having optimized the expression process at small scale, we understand the pitfalls of upscaling. We ensure a smooth transition to producing large quantities of your target protein without compromising quality.
