Recombinant Protein Expression in E.coli

Our comprehensive Protein Expression in E.coli Service Package covers everything from cloning to high-purity protein purification. With extensive expertise in bacterial protein expression, we deliver reliable support for your research—whether it’s a standard protein or a challenging target.

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Service icon for Recombinant Protein Expression in E.coli_trenzyme GmbH. The icon shows an E. coli bacterium.

Recombinant Protein Expression in E.coli – Service Package

Final protein yield starting at 1 mg with >90% purity
Complete workflow – from cloning to purified protein – in just 4 weeks
Reliable data and process foundation for future upscale and customized solutions
Available with high-throughput and large-scale production capabilities
High quality service for Recombinant Protein Expression in E.coli

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Workflow of Bacterial Protein Expression (E.coli)

Recombinant-Protein-Expression-Service-in-E.coli_Process-Steps_trenzyme-GmbH

Process of Recombinant Protein Expression in E.coli

Planning Phase

Planning Phase

After submitting your inquiry via contact form, email, or phone, a member of our scientific sales team will get back to you within one business day to discuss your project requirements. You will then receive a quotation, and your project can begin within one day after order confirmation.

Plasmid Generation

Plasmid Generation

Your target sequence(s) will be cloned into the selected expression system or vector(s) as defined during the quotation process. Plasmid DNA containing codon-optimized POI sequences is either provided by the client or generated via gene synthesis (optional add-on).

Small Scale Pilot Study

Small Scale Pilot Study

Before initiating full-scale production, we assess soluble expression of the target protein in E. coli and evaluate purification feasibility. For more details, please refer to our FAQs.

Protein Expression

Protein Expression

The protein is expressed in shake flask culture, for larger scales, fed-batch fermentation is also possible.

Protein Purification

Protein Purification

Based on the pilot study results, the target protein is purified using either a one-step or multistep approach. The buffer is subsequently exchanged into a standard buffer or a customer-defined formulation. The full amount of purified protein obtained during production is delivered to the client.

Final Quality Check

Final Quality Check & Further Analytics

Before delivery of your target protein, a final quality control step is performed. This includes standard analysis by SDS-PAGE as well as determination of concentration and purity, documentation of the complete production process, and optional additional analyses upon request (see FAQs).

Shipment of Your Protein

Shipment of Your Protein

Priority courier shipping to destinations worldwide
Secure and reliable delivery to maintain protein integrity
Established network of trusted logistics partners
Real-time tracking for full shipment transparency

For more information, please refer to our FAQs.

Deliverables & Timeline

Deliverables & Timeline

Timeline: The duration of the process typically takes between 4 and 6 weeks.

Deliverables: Along with the purified target protein, you will receive comprehensive documentation.

For further details, please refer to our FAQs.

What Our Clients Say About Us

“(…) trenzyme has been a reliable, flexible, and innovative outsourcing partner. (…)”

Dr. C. Smerling, 3B Pharmaceuticals GmbH

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trenzyme's Protein Service includes the following points. Open Hand with vial.

Recombinant Protein Expression in E.coli – Service Package for Standard Protein Targets Includes

All steps from cloning to purification
Final yield: starting at 1 mg purified protein (more on request)
Final purity: > 90 %
Short processing time (for standard proteins): 4 business weeks including cloning, expression, purification and QC
As starting material we can either use an existing expression vector construct from the client or start with codon-optimized gene synthesis (additional service option).
Free initial consultation on your individual project by our experienced protein experts at PhD level
Small scale pilot study for evaluation of manufacturability
Delivery of test samples from pilot study if desired
Use of an optimized and reliable E coli protein expression system
Comprehensive datasheet

Additional Service Options:

➥Gene synthesis and ➥High-Throughput Protein Expression Screening as starting point
Purchase of additional protein produced by our E. coli recombinant protein production service
Extended Quality Control: e.g. mass spectrometry, endotoxin detection, Western Blot or ELISA
Low endotoxin purification and endotoxin removal
➥Custom Protein Production Service for larger protein quantities, challenging targets or more specific project requirements

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trenzyme’s E. coli Recombinant Protein Production Service – Your Benefits

Comprehensive Service Package

Comprehensive service package for recombinant protein expression and purification.

Transparency & High Quality

Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.

Free Feasibility Analysis & Consulting

Our free feasibility check of your protein sequence is the basis for our free individual consultation with you to define the ideal service strategy for your target that will lead to success.

Standard Protein or Challenging Target – Evaluated by Feasibility Check and Pilot Study

During initial free individual consulting, our scientific experts run a feasibility check (e.g. literature check, database check, product check, etc.) with your protein sequence to define the ideal service strategy for your specific target.

To achieve maximum efficiency and save you significant time and money, we additionally run a small scale pilot study in this service process. These preliminary assessments help us determine in advance whether the target protein expression in E.coli is possible and whether the purification process is feasible. If desired, we will provide you with test samples to check the functionality and quality of the purified target protein in your hands.

The big benefit for you: After pilot scale evaluation we guarantee the final yield, purity and timeline – you only pay the production run when we succeed!

If your target sequence is determined as challenging protein or you requested a high yield production (> 10 mg), we offer highly modular and ➥custom-tailored solutions.

Protein Expression and Purification Service_Decision Path

Recombinant Protein Expression Service in E.coli
Application Note: Recombinant expression of ¹⁵N-labeled protein in E.coli by high cell density cultivation

Preview of trenzyme´s Application Note: Recombinant Protein Expression in E.coli

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FAQs for Recombinant Protein Expression in E.coli

Can you provide a corresponding antibody for a recombinant protein?

If you need both, your recombinant protein and a corresponding antibody, our guaranteed antibody package will be the best service choice for you.

How will proteins expressed in E.coli be shipped?

Our standard priority shipment will be on dry ice. If required, we offer shipment on blue ice, at -20°C or even at room temperature (lyophilized proteins only) as well.

Are recombinant proteins and antibodies sterile?

We can additionally offer sterile filtration if you require this service.

What are the advantages of protein expression in E.coli compared to other expression systems?

E.coli is a widely used host for protein production due to its cost-effectiveness, especially when scaling up to larger production volumes. The expression processes are typically fast, straightforward and can be easily and economically scaled up. This makes E.coli an efficient choice for many applications.

Can eukaryotic targets be expressed in E.coli?

Yes, eukaryotic proteins can be synthesized in E.coli. However, certain limitations may arise due to the need for post-translational modifications such as glycosylation, complex folding processes and the formation of physiologically correct disulfide bonds. These factors can impact the successful production of a functional target protein.

Is secretion of a target protein into the periplasm possible in E.coli, and what are the benefits?

One key advantage of E.coli as a Gram-negative bacterial host is its ability to secrete proteins into the periplasmic space, which provides an oxidizing environment that promotes the correct formation of disulfide bonds. This can enhance proper protein folding and solubility, making it a cost-effective and efficient strategy for producing structurally complex or even eukaryotic proteins in E. coli. Additionally, periplasmic secretion can be a promising approach for expression of challenging or potentially toxic targets.

Is co-expression of multiple proteins possible in E.coli?

Yes, co-expression in E.coli allows the production of heterodimers, oligomers, and multiprotein complexes, enabling the synthesis of more complex target architectures. Furthermore, co-expression of enzymatically active interaction partners can facilitate specific modifications, such as targeted biotinylation of recombinantly produced proteins. The co-expression of molecular chaperones or folding helpers can also improve the solubility and stability of complex target molecules.

See General FAQs

reinhold-horlacher-managing-director-trenzyme

Reinhold Horlacher, PhD

Managing Director & CSO

We would be happy to provide you with support on your protein research project.

Get In Contact With Our Scientific Experts

Design Your Protein Now!

Use our user-friendly protein configurator to create your individual protein according to your needs. Click on the button below to get started!

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Questions or Individual Quote?

Let us know your questions and requests by simply filling out the following contact form. Our scientific experts will get in touch with you immediately to discuss your request.

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