Recombinant Protein Expression in E.coli (Escherichia coli)

Our comprehensive service package comprises all steps from cloning to recombinant protein expression in E.coli (Escherichia coli) and high quality protein purification of your target protein. Our extensive expertise in bacterial protein expression enables us to provide you with the best possible support for your research project, whether it is a standard protein or a challenging target.

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Service icon for Recombinant Protein Expression in E.coli_trenzyme GmbH. The icon shows an E. coli bacterium.

Recombinant Protein Expression in E.coli – Service Package for Standard Proteins

Our recombinant protein expression in E.coli (Escherichia coli) service package is ideal for standard proteins. It includes all steps from cloning to final protein purification with a guaranteed final yield starting at 1 mg and high protein purity of > 90% in a very short processing time of 4 business weeks. If you need limited quantities of a particular target protein, e.g. for initial target characterization or early-stage production, this is the service of choice.

Furthermore, manufacturability and production data gained in the framework of this E.coli recombinant protein production service package can also serve as a valuable starting point for future, upscaled and/or refined production of your target protein at trenzyme.

Do you have a challenging protein target with specific project requirements? Please contact us if you need larger quantities or have additional project requirements related to our E.coli recombinant protein production service. Our highly qualified protein experts are happy to assist you and offer highly customized solutions with our ➥Custom Protein Production Service.

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Process of Recombinant Protein Expression in E.coli

Recombinant-Protein-Expression-Service-in-E.coli_Process-Steps_trenzyme-GmbH

trenzyme's Protein Service includes the following points. Open Hand with vial.

Recombinant Protein Expression in E.coli – Service Package for Standard Protein Targets Includes

All steps from cloning to purification
Final yield: starting at 1 mg purified protein (more on request)
Final purity: > 90 %
Short processing time (for standard proteins): 4 business weeks including cloning, expression, purification and QC
As starting material we can either use an existing expression vector construct from the client or start with codon-optimized gene synthesis (additional service option).
Free initial consultation on your individual project by our experienced protein experts at PhD level
Small scale pilot study for evaluation of manufacturability
Delivery of test samples from pilot study if desired
Use of an optimized and reliable E coli protein expression system
Comprehensive datasheet

Additional Service Options:

➥Gene synthesis and ➥High-Throughput Protein Expression Screening as starting point
Purchase of additional protein produced by our E. coli recombinant protein production service
Extended Quality Control: e.g. mass spectrometry, endotoxin detection, Western Blot or ELISA
Low endotoxin purification and endotoxin removal
➥Custom Protein Production Service for larger protein quantities, challenging targets or more specific project requirements

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trenzyme’s E. coli Recombinant Protein Production Service – Your Benefits

Guarantee – Pay Only When We Succeed!

After feasibility analysis and sucessful pilot study we guarantee the final quantity and purity of your desired target protein in the given timeframe. You only pay when we succeed!

Comprehensive Service Package

Comprehensive service package for recombinant protein expression and purification for a fixed price and a fixed scale.

Transparency & High Quality

Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.

Free Feasibility Analysis & Consulting

Our free feasibility check of your protein sequence is the basis for our free individual consultation with you to define the ideal service strategy for your target that will lead to success.

Design Your Protein Now!

What Our Clients Say About Us

“(…) trenzyme has been a reliable, flexible, and innovative outsourcing partner. (…)”

Dr. C. Smerling, 3B Pharmaceuticals GmbH

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Standard Protein or Challenging Target –Evaluated by Feasibility Check and Pilot Study

During initial free individual consulting, our scientific experts run a feasibility check (e.g. literature check, database check, product check, etc.) with your protein sequence to define the ideal service strategy for your specific target.

To achieve maximum efficiency and save you significant time and money, we additionally run a small scale pilot study in this service process. These preliminary assessments help us determine in advance whether the target protein expression in E.coli is possible and whether the purification process is feasible. If desired, we will provide you with test samples to check the functionality and quality of the purified target protein in your hands.

The big benefit for you: After pilot scale evaluation we guarantee the final yield, purity and timeline – you only pay the production run when we succeed!

If your target sequence is determined as challenging protein or you requested a high yield production (> 10 mg), we offer highly modular and ➥custom-tailored solutions.

Protein Expression and Purification Service_Decision Path

FAQs for Recombinant Protein Expression in E.coli

Can you provide a corresponding antibody for a recombinant protein?

If you need both, your recombinant protein and a corresponding antibody, our guaranteed antibody package will be the best service choice for you.

How will proteins expressed in E.coli be shipped?

Our standard priority shipment will be on dry ice. If required, we offer shipment on blue ice, at -20°C or even at room temperature (lyophilized proteins only) as well.

Are recombinant proteins and antibodies sterile?

We can additionally offer sterile filtration if you require this service.

What are the advantages of protein expression in E.coli compared to other expression systems?

E.coli is a widely used host for protein production due to its cost-effectiveness, especially when scaling up to larger production volumes. The expression processes are typically fast, straightforward and can be easily and economically scaled up. This makes E.coli an efficient choice for many applications.

Can eukaryotic targets be expressed in E.coli?

Yes, eukaryotic proteins can be synthesized in E.coli. However, certain limitations may arise due to the need for post-translational modifications such as glycosylation, complex folding processes and the formation of physiologically correct disulfide bonds. These factors can impact the successful production of a functional target protein.

Is secretion of a target protein into the periplasm possible in E.coli, and what are the benefits?

One key advantage of E.coli as a Gram-negative bacterial host is its ability to secrete proteins into the periplasmic space, which provides an oxidizing environment that promotes the correct formation of disulfide bonds. This can enhance proper protein folding and solubility, making it a cost-effective and efficient strategy for producing structurally complex or even eukaryotic proteins in E. coli. Additionally, periplasmic secretion can be a promising approach for expression of challenging or potentially toxic targets.

Is co-expression of multiple proteins possible in E.coli?

Yes, co-expression in E.coli allows the production of heterodimers, oligomers, and multiprotein complexes, enabling the synthesis of more complex target architectures. Furthermore, co-expression of enzymatically active interaction partners can facilitate specific modifications, such as targeted biotinylation of recombinantly produced proteins. The co-expression of molecular chaperones or folding helpers can also improve the solubility and stability of complex target molecules.

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reinhold-horlacher-managing-director-trenzyme

Reinhold Horlacher, PhD

Managing Director & CSO

We would be happy to provide you with support on your protein research project.

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Design Your Protein Now!

Use our user-friendly protein configurator to create your individual protein according to your needs. Click on the button below to get started!

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Questions or Individual Quote?

Let us know your questions and requests by simply filling out the following contact form. Our scientific experts will get in touch with you immediately to discuss your request.

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