Custom Protein Expression Service

Our experienced scientific specialists provide high quality recombinant protein expression and purification services tailored to your specific needs. Whether you are interested in standard recombinant protein production or highly customized solutions for your challenging target, as your trusted research partner we are committed to providing the ideal service strategy for your research project and delivering high quality proteins to accelerate your innovation. Our experts have extensive experience in using different hosts (E.coli, insect cells, yeast, mammalian cells, etc.) to express your gene of interest and we’re fully equipped to handle them.

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Custom Protein Expression Service_trenzyme GmbH

Custom Protein Expression Service

Make Your Research Project a Success With trenzymes’ Experienced Protein Department

We offer a one-stop-shop for your individual target: from gene synthesis and cloning to high quality purification. All we need to start production and purification of your recombinant proteins is your target sequence. Our scientific experts have a vast knowledge on all current expression platforms, including bacteria, yeast, insect and mammalian cells, based on our longstanding experience.

Individual consulting and a free feasibility analysis for every project ensure that we follow the best strategy to produce your target protein. We at trenzyme set great value in high quality work, reliability and transparency. Our dedicated project management and our scientific experts will be in close communication with you to ensure a successful project progression and to react with high flexibility to your individual demands. We look forward to making your research project a success.

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Custom Protein Expression Service

Highly Customized Solution for Your Target Expression

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We provide free individual consulting and modular systems to develop a highly customized and flexible project strategy that is tailored to your needs. We work in close collaboration with our clients to ensure high quality and efficiency. Our dedicated project management will guide your project through every step of the process and keep you constantly informed about the project progress via continued status reports. Due to the constantly exchange of information between you and our scientific experts, we react with highest flexibility to potential project strategy changes.

Protein Expression System Selection Guide

for Your Target Expression

We provide free individual consulting and modular systems to develop a highly customized and flexible project strategy that is tailored to your needs. We work in close collaboration with our clients to ensure high quality and efficiency. Our dedicated project management will guide your project through every step of the process and keep you constantly informed about the project progress via continued status reports. Due to the constantly exchange of information between you and our scientific experts, we react with highest flexibility to potential project strategy changes.

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Contact Us

Your Benefits

From Working With Us

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Customization

Our modular service packages are custom-tailored to your project. We provide you highly customized and flexible strategies for your recombinant protein expression and purification project.

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Transparency & Communication

Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.

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Longstanding Expertise

With longstanding expertise on recombinant protein expression and a vast knowledge base on various expression host organisms, we are able to complete your research project successfully and efficiently.

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High Quality Standards

As an ISO-certified company working with a LIMS based documentation, all projects are completed following highest standards.

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Overview About Our Custom Protein Production Services

Highly Customized, Flexible and Modular

Icon Recombinant Protein Expression Service_trenzyme GmbH

Recombinant Protein Expression Services

  • Extensive knowledge on different expression systems: E.coli, insect cells, yeast and mammalian cells
  • High expression yields [mg-g] for production in bacteria (E. coli), yeast and mammalian cells possible
  • Collection of more than > 300 ready-to-go expression vectors (vector toolbox)
  • Process development and upscaling according to your individual project requirements
  • Expression host screening and productivity screening
  • Optimization of recombinant protein expression
  • Optimization of soluble protein production
Recombinant Protein Purification Services

Recombinant Protein Purification Services

  • State-of-the-art purification strategies
  • Optimization of purification process
  • Refolding of denatured proteins
  • Endotoxin free purification or endotoxin removal
  • Protein characterization
  • Extended quality control according to your needs
  • Implementation of activity assay
Custom Protein Expression Service_trenzyme GmbH

General Custom Protein Production Services

  • Continued feedback about project process leads to maximum project success
  • Extended optional feasibility analysis according to your specific research project requirements
  • Gene synthesis with codon optimization as starting point if desired
  • DNA cloning into your desired vector or into our proprietary expression vector saves time and money
  • Highly individualized and flexible strategies for your recombinant protein expression and purification
  • Extensive services for challenging proteins
  • Process development including comprehensive report
  • Detailed documentation of all project steps by a Laboratory Information Management System (LIMS)
  • High quality standards (DIN EN ISO 9001:2015 certified company)

What Our Clients Say About Us

Due to the outstanding flexibility to address our particular project needs as well as the excellent quality of the provided services, trenzyme is one of our preferred service providers since many years.

Dr. Steffen Schaffer Evonik Creavis GmbH, Marl, Germany
EVONIK INDUSTRIES Logo

Relevant Publications of trenzyme’s Highly Qualified Scientific Team

Growth hormone isoform-differential mass spectrometry for doping control purposes. Drug Test. Anal. 10, 938–946. (2018)

Arsene C, Schulze D, Röthke A, Thevis M, and Henrion A

 

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Protein phosphatase V ensures timely cell cycle remodeling during the mid-blastula transition in Drosophila. bioRxiv 172379. (2017)

Liu B, Sung H, Gregor I, Müller A, and Grosshans J

 

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An internally modulated, thermostable, pH-sensitive Cys loop receptor from the hydrothermal vent worm Alvinella pompejana. J. Biol. Chem. 289, 15130–15140. (2014)

Juneja P, Horlacher R, Bertrand D, Krause R, Marger F, and Welte W

 

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Role of periplasmic trehalase in uptake of trehalose by the thermophilic bacterium Rhodothermus marinus. J. Bacteriol. 190, 1871–1878. (2008)

Jorge CD, Fonseca LL, Boos W, and Santos H

 

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A highly thermostable trehalase from the thermophilic bacterium Rhodothermus marinus. Extrem. Life Extreme Cond. 11, 115–122. (2007)

Jorge CD, Sampaio MM, Hreggvidsson GO, Kristjánson JK, and Santos H

 

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The high-affinity maltose/trehalose ABC transporter in the extremely thermophilic bacterium Thermus thermophilus HB27 also recognizes sucrose and palatinose. J. Bacteriol. 187, 1210–1218. (2005)

Silva Z, Sampaio M-M, Henne A, Böhm A, Gutzat R, Boos W, da Costa MS, and Santos H

 

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The crucial role of trehalose and structurally related oligosaccharides in the biosynthesis and transfer of mycolic acids in Corynebacterineae. J. Biol. Chem. 280, 26573–26585. (2005)

Tropis M, Meniche X, Wolf A, Gebhardt H, Strelkov S, Chami M, Schomburg D, Krämer R, Morbach S, and Daffé M

 

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TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis. J. Biol. Chem. 279, 47890–47897. (2004)

Qu Q, Lee S-J, and Boos W

 

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The YadA protein of Yersinia pseudotuberculosis mediates high-efficiency uptake into human cells under environmental conditions in which invasin is repressed. Infect. Immun. 70, 4880–4891. (2002)

Eitel J, and Dersch P

 

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Custom Protein Expression Service FAQs

What kind of analysis of the produced proteins do you offer?

We offer different analytical methods according to your needs. Always included is SDS-PAGE and determination of protein concentration. Very often we do Western blot analysis. If needed, we can determine the oligomeric state by analytical size exclusion chromatography (SEC) or native PAGE. Mass spectroscopic analysis is also offered on request to determine for example the modification of proteins. Other assays such as ELISA and activity assays are available.

Does trenzyme offer recombinant proteins off the shelf?

Yes, we offer various recombinant proteins off the shelf. Have a look at our continuously growing catalog.

How many custom proteins have you produced so far? What is the typical success rate?

We are working on recombinant proteins for more than 20 years and we have successfully produced more than 3000 different proteins so far.

Which protein classes can you produce?

Our expertise covers a wide range of protein classes, including soluble enzymes and secreted proteins such as antibodies and their derivatives, as well as more complex variants such as nuclear and membrane proteins. To ensure the ultimate success for your target protein, we identify and utilize the most appropriate expression system and backbones and screen different variants in small scale.

What is the concentration range of your proteins? How do you determine the quantity of them?

The concentration is mostly between 0.1 and 2 mg/ml but can be adjusted according to your needs. The concentration will be determined by either BCA, adsorption at 280nm or densitometric determination after Coomassie staining.

Can tags be cleaved off from recombinant proteins?

Yes, in most cases we use cleavable tag sequences. But cleavage efficiency can be influenced by the target protein and has to be tested.

Which countries does trenzyme deliver to?

  • Worldwide shipping subject to Embargo
  • Short delivery times
  • Our logistic experts and our delivery partners ensure that all products arrive to you in a perfect state without any loss of quality
  • Trusted and well-known delivery partners operating a worldwide logistics system
  • Continuous tracking and tracing of parcels (the tracking code is made available to you at the time of despatch)
  • Proteins/cell lines are usually shipped on dry ice to prevent quality loss during shipping. If required we can also organize alternative shipping conditions ( e.g. dry shipper, blue ice or room temperature)
  • We are committed to delivering our products with care, as sustainable environmental and climate protection is an important corporate objective (see Code of Conduct).
See General FAQs
Reinhold Horlacher CEO trenzyme
Reinhold Horlacher, PhD
Managing Director & CSO

We would be happy to provide you with support on your protein research project. Let us know your questions and requests, our scientific experts will reply shortly.

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With Our Scientific Experts

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