Gene Synthesis and Custom Cloning

High quality recombinant proteins and stable cell lines require high quality DNA. Whether you are interested in gene synthesis, DNA cloning or mutagenesis – our molecular biology department delivers the ideal recombinant gene or plasmid tools for the downstream applications that accelerate your life science innovation.

Learn more

Gene Synthesis

Fast delivery, direct cloning possible
High quality synthetic genes “Made in Germany”
Free codon and DNA structure optimization – optimize DNA sequences to significantly increase protein expression in any host
No limitations – any sequence in any vector. Accurately and efficiently we fuse your gene into any site on any vector
Collection of various expression vectors instantly available (vector toolbox)

Simply provide us with your desired target sequence and we will take care of the rest. Every synthesis service includes free codon and DNA structure optimization, if desired. We additionally offer comprehensive downstream services that accelerate your research as DNA subcloning into your own expression vector or in one of our huge vector collection.

Custom Cloning

Highly individual complex cloning projects possible
Huge collection of various expression vectors instantly available (vector toolbox)
Guaranteed results

Whether you need DNA cloning of PCR fragments, seamless cloning, sub-cloning, mutagenesis, plasmid purification or general vector construction, we are your one-stop-shop for uncompromising high quality plasmid construction.

Our molecular biology experts can bundle gene synthesis with cloning into the vector of your choice, or you can order DNA cloning projects from templates (plasmid or genomic DNA) you already have. Every other and even complex cloning strategy is possible – ask our experts, we will deliver the best cloning strategy for your individual needs.

Simply provide us with your electronic sequences or your project outline – we will propose a customized quotation.

highly individual complex cloning projects

Additional Services

➥From gene to cell line – development of stable recombinant cell lines with constitutive or inducible target expression.
➥From gene to protein – recombinant protein production and purification services. From standard to highly customized solutions.
➥From gene to manufacturing – development of GMP-compliant high producer cell lines suitable for use from the R&D phase up to successful introduction of your product to the market.

Take your research project to the next level with our tailor-made services for ➥recombinant protein production and for ➥stable cell line development (GMP or non-GMP). Let us know your questions and requests about our DNA synthesis service, we will contact you shortly and provide the ideal solution for your project.

Relevant Publications

Cytokine-induced interleukin-1 receptor antagonist protein expression in genetically engineered equine mesenchymal stem cells for osteoarthritis treatment. J. Gene Med. 20, e3021. (2018)

Gabner S, Ertl R, Velde K, Renner M, Jenner F, Egerbacher M, and Hlavaty J

Gene Synthesis and Custom Cloning

Gene Synthesis

Custom Cloning

Additional Services

Relevant Publications

Cytokine-induced interleukin-1 receptor antagonist protein expression in genetically engineered equine mesenchymal stem cells for osteoarthritis treatment. J. Gene Med. 20, e3021. (2018)

Protein engineering of recombinant human bone morphogenetic protein 2 with higher interaction with Ca phosphate based scaffold used for osteogenesis. J. Biomed. Mater. Res. A 105, 2799–2805. (2017)

Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii. PloS One 8, e53440. (2013)

Structural and mechanistic studies of pesticin, a bacterial homolog of phage lysozymes. J. Biol. Chem. 287, 23381–23396. (2012)

Physical and functional interaction of the HECT ubiquitin-protein ligases E6AP and HERC2. J. Biol. Chem. 286, 19410–19416. (2011)

Exploring the Active Site of the Tungsten, Iron-Sulfur Enzyme Acetylene Hydratase. J. Bacteriol. 193, 1229–1236. (2011)

Unequal allelic expression of wild-type and mutated β-myosin in familial hypertrophic cardiomyopathy. Basic Res. Cardiol. 106, 1041–1055. (2011)

Development of a second version of the Cobas AmpliPrep/Cobas TaqMan hepatitis C virus quantitative test with improved genotype inclusivity. J. Clin. Microbiol. 49, 3309–3315. (2011)

Crystallization and preliminary X-ray data collection of the Escherichia coli lipoproteins BamC, BamD and BamE. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 66, 1586–1590. (2010)

TLR2 mediates gap junctional intercellular communication through connexin-43 in intestinal epithelial barrier injury. J. Biol. Chem. 284, 22332–22343. (2009)

Expression and purification of neurolin immunoglobulin domain 2 from Carrassius auratus (goldfish) in Escherichia coli. Protein Expr. Purif. 59, 47–54. (2008)

The bifunctional flavokinase/flavin adenine dinucleotide synthetase from Streptomyces davawensis produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin. J. Bacteriol. 190, 1546–1553. (2008)

Effect of macrophage overexpression of murine liver X receptor-alpha (LXR-alpha) on atherosclerosis in LDL-receptor deficient mice. Arterioscler. Thromb. Vasc. Biol. 28, 2009–2015. (2008)

Characterization of the TrmB-like protein, PF0124, a TGM-recognizing global transcriptional regulator of the hyperthermophilic archaeon Pyrococcus furiosus. Mol. Microbiol. 65, 305–318. (2007)

Stability of DNA repeats in Escherichia coli dam mutant strains indicates a Dam methylation-dependent DNA deletion process. Gene 258, 95–108. (2000)

A continuous coupled enzyme assay for bacterial malonyl-CoA:acyl carrier protein transacylase (FabD). Anal. Biochem. 319, 171–176. (2003)

Reinhold Horlacher, PhD

Get In Contact

With Our Scientific Experts

Enquire now