Gene Synthesis and Custom Cloning

High quality recombinant proteins and stable cell lines require high quality DNA. Whether you are interested in gene synthesis, DNA cloning or mutagenesis – our molecular biology department delivers the ideal recombinant gene or plasmid tools for the downstream applications that accelerate your life science innovation.

woman with pipette and test tubes

Gene Synthesis

  • Fast delivery, direct cloning possible
  • High quality synthetic genes “Made in Germany”
  • Free codon and DNA structure optimization – optimize DNA sequences to significantly increase protein expression in any host
  • No limitations – any sequence in any vector. Accurately and efficiently we fuse your gene into any site on any vector
  • Collection of various expression vectors instantly available (vector toolbox)

Simply provide us with your desired target sequence and we will take care of the rest. Every synthesis service includes free codon and DNA structure optimization, if desired. We additionally offer comprehensive downstream services that accelerate your research as DNA subcloning into your own expression vector or in one of our huge vector collection.

Custom Cloning

  • Highly individual complex cloning projects possible
  • Huge collection of various expression vectors instantly available (vector toolbox)
  • Guaranteed results

Whether you need DNA cloning of PCR fragments, seamless cloning, sub-cloning, mutagenesis, plasmid purification or general vector construction, we are your one-stop-shop for uncompromising high quality plasmid construction.

Our molecular biology experts can bundle gene synthesis with cloning into the vector of your choice, or you can order DNA cloning projects from templates (plasmid or genomic DNA) you already have. Every other and even complex cloning strategy is possible – ask our experts, we will deliver the best cloning strategy for your individual needs.

Simply provide us with your electronic sequences or your project outline – we will propose a customized quotation.

highly individual complex cloning projects
trenzymes scientific experts pipette

Additional Services

  • From gene to cell line – development of stable recombinant cell lines with constitutive or inducible target expression.
  • From gene to protein – recombinant protein production and purification services. From standard to highly customized solutions.
  • From gene to manufacturing – development of GMP-compliant high producer cell lines suitable for use from the R&D phase up to successful introduction of your product to the market.

Take your research project to the next level with our tailor-made services for recombinant protein production and for stable cell line development (GMP or non-GMP). Let us know your questions and requests about our DNA synthesis service, we will contact you shortly and provide the ideal solution for your project.

Relevant Publications

Cytokine-induced interleukin-1 receptor antagonist protein expression in genetically engineered equine mesenchymal stem cells for osteoarthritis treatment. J. Gene Med. 20, e3021. (2018)

Gabner S, Ertl R, Velde K, Renner M, Jenner F, Egerbacher M, and Hlavaty J


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Protein engineering of recombinant human bone morphogenetic protein 2 with higher interaction with Ca phosphate based scaffold used for osteogenesis. J. Biomed. Mater. Res. A 105, 2799–2805. (2017)

Bayat M, Shojaei S, Bahrami N, Mohamadnia A, Shojaei P, and Bahrami N


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Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii. PloS One 8, e53440. (2013)

Frangoulidis D, Splettstoesser WD, Landt O, Dehnhardt J, Henning K, Hilbert A, Bauer T, Antwerpen M, Meyer H, Walter MC, et al.


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Structural and mechanistic studies of pesticin, a bacterial homolog of phage lysozymes. J. Biol. Chem. 287, 23381–23396. (2012)

Patzer SI, Albrecht R, Braun V, and Zeth K


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Physical and functional interaction of the HECT ubiquitin-protein ligases E6AP and HERC2. J. Biol. Chem. 286, 19410–19416. (2011)

Kühnle S, Kogel U, Glockzin S, Marquardt A, Ciechanover A, Matentzoglu K, and Scheffner M


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Exploring the Active Site of the Tungsten, Iron-Sulfur Enzyme Acetylene Hydratase. J. Bacteriol. 193, 1229–1236. (2011)

tenBrink F, Schink B, and Kroneck PMH


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Unequal allelic expression of wild-type and mutated β-myosin in familial hypertrophic cardiomyopathy. Basic Res. Cardiol. 106, 1041–1055. (2011)

Tripathi S, Schultz I, Becker E, Montag J, Borchert B, Francino A, Navarro-Lopez F, Perrot A, Özcelik C, Osterziel K-J, et al.


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Development of a second version of the Cobas AmpliPrep/Cobas TaqMan hepatitis C virus quantitative test with improved genotype inclusivity. J. Clin. Microbiol. 49, 3309–3315. (2011)

Vermehren J, Colucci G, Gohl P, Hamdi N, Abdelaziz AI, Karey U, Thamke D, Zitzer H, Zeuzem S, and Sarrazin C


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Crystallization and preliminary X-ray data collection of the Escherichia coli lipoproteins BamC, BamD and BamE. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 66, 1586–1590. (2010)

Albrecht R, and Zeth K


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TLR2 mediates gap junctional intercellular communication through connexin-43 in intestinal epithelial barrier injury. J. Biol. Chem. 284, 22332–22343. (2009)

Ey B, Eyking A, Gerken G, Podolsky DK, and Cario E


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Expression and purification of neurolin immunoglobulin domain 2 from Carrassius auratus (goldfish) in Escherichia coli. Protein Expr. Purif. 59, 47–54. (2008)

Drees C, Stürmer CAO, Möller HM, and Fritz G


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The bifunctional flavokinase/flavin adenine dinucleotide synthetase from Streptomyces davawensis produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin. J. Bacteriol. 190, 1546–1553. (2008)

Grill S, Busenbender S, Pfeiffer M, Köhler U, and Mack M


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Effect of macrophage overexpression of murine liver X receptor-alpha (LXR-alpha) on atherosclerosis in LDL-receptor deficient mice. Arterioscler. Thromb. Vasc. Biol. 28, 2009–2015. (2008)

Teupser D, Kretzschmar D, Tennert C, Burkhardt R, Wilfert W, Fengler D, Naumann R, Sippel AE, and Thiery J


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Characterization of the TrmB-like protein, PF0124, a TGM-recognizing global transcriptional regulator of the hyperthermophilic archaeon Pyrococcus furiosus. Mol. Microbiol. 65, 305–318. (2007)

Lee S-J, Surma M, Seitz S, Hausner W, Thomm M, and Boos W


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Stability of DNA repeats in Escherichia coli dam mutant strains indicates a Dam methylation-dependent DNA deletion process. Gene 258, 95–108. (2000)

Troester H, Bub S, Hunziker A, and Trendelenburg MF


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A continuous coupled enzyme assay for bacterial malonyl-CoA:acyl carrier protein transacylase (FabD). Anal. Biochem. 319, 171–176. (2003)

Molnos J, Gardiner R, Dale GE, and Lange R


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Reinhold Horlacher CEO trenzyme
Reinhold Horlacher, PhD
Managing Director & CSO

We would be happy to provide you with support on your protein research project. Let us know your questions and requests, our scientific experts will reply shortly.

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