Stem Cell Services

With extensive expertise in hiPSC technology, trenzyme offers a comprehensive range of stem cell services tailored to meet your research needs. Our services include hiPSC characterization and precise directed differentiation into functional cell types across multiple lineages. At trenzyme, we support your stem cell research with customized solutions and excellence in hiPSC-based workflows.

Stem Cell Differentiation

Differentiation into all cell types (endo-, meso-, or ectoderm lineages). Our expert team serves as an extended workbench for your iPSC differentiation needs, specializing in differentiating hiPSCs into diverse cell types like cardiomyocytes, neurons, microglia, hepatocytes and monocytes. Choose from our proven protocols or send us your iPSCs and SOPs, and we’ll handle the full technology transfer and cell production for you.

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Stem Cell Characterization

Variety of iPSC culture formats and functional readouts are available. Reliable cell models for drug discovery and preclinical research require an extensive characterization of the generated hiPSC. Our expert team ensures this with comprehensive services, including trilineage differentiation to confirm pluripotency and advanced marker analysis via RT-qPCR, immunostaining, and flow cytometry.

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stem cell characterization service

Stem Cell Characterization Service

Characterization of human induced Pluripotent Stem Cells (hiPSC)

Potential of hiPSC Technology: The field of human induced pluripotent stem cell (hiPSC) technology hold significant potential for applications in drug discovery, toxicology testing, and regenerative medicine.
Challenges in the Reprogramming Process: Since the first hiPSCs were created, various reprogramming methods have been developed. During reprogramming the cells might suffer from cellular stress and epigenetic missregulations. Both could alter the cellular phenotype in terms of pluripotency and impacting experimental reliability.
Validation Procedures at trenzyme: To overcome these challenges it is essential to establish robust molecular and functional assays to verify pluripotency and detect any genetic abnormalities from reprogramming. trenzyme routinely validates hiPSC lines by assessing pluripotency markers (via ICC and qPCR), genetic stability (molecular karyotyping), and most importantly differentiation potential (trilineage differentiation).

stem cell differentiation service

Stem Cell Differentiation Service

Differentiation of human induced Pluripotent Stem Cells (hiPSC)

hiPSCs as Advanced Disease Models: Once differentiated into specific cell types such as neurons, cardiomyocytes, or hepatocytes, hiPSCs serve as sophisticated “disease-in-a-dish” models, ideal for applications in drug discovery, toxicology, basic research, and preclinical development.
Overcome Limitations of Conventional Cell Models: Many current applications in academia and industry rely on cancer cell lines or primary cells, which have drawbacks like impaired functionality, availability, and donor variability. hiPSCs, which can be derived consistently from healthy or disease-specific tissue, offer a significant advantage over traditional cell lines and primary cells by avoiding issues like impaired functionality, limited availability, and variability.
Advantages of hiPSCs Over Other Cell Models: hiPSCs offer unlimited expansion potential and can differentiate into all cell types from the three germ layers (endo-, meso-, ectoderm) without the limitations of other cell models, especially useful for cell types that are not available from human tissue such as neurons or cardiomyctes that are if so only available post-mortem.
Differentiation Service at trenzyme: trenzyme leverages its deep expertise in human induced Pluripotent Stem Cells (hiPSCs) to advance your research projects by providing reliable, flexible, and physiologically relevant model systems.

What Our Clients Say About Us

trenzyme is one of our preferred service providers for cell line development and molecular biology services. The impressive level of personal customer liaison and the scientific competence are decisive factors for us to cooperate with trenzyme for many years and even to intensify in the area of demanding iPS cell line projects.

Dr. Margit Bauer & Dr. Ralf Heilker Boehringer Ingelheim Pharma GmbH & Co KG, Biberach, Germany

stem cell services_trenzyme GmbH

Stem Cell Services

Background Information on human induced Pluripotent Stem Cells (hiPSC)

hiPSCs can be generated from somatic cells of healthy donors or patient-specific disease samples. Once differentiated into tissue-specific cells, such as neurons, cardiomyocytes, or hepatocytes, hiPSCs act as advanced models, supporting a wide range of applications in basic research, drug discovery, toxicology and preclinical development.

Currently, many applications in academia, biotech, and pharma still depend on immortalized cancer cell lines, which come with significant limitations, including abnormal gene expression, and dysfunctional signaling pathways. Even human primary cells face serious issues, such as donor variability, and practical limitations like restricted availability and expansion.

In contrast, hiPSCs avoid these challenges, offering unlimited expansion potential and the ability to differentiate into any cell type from the three germ layers (ectoderm, mesoderm, endoderm). Additionally, intermediate stages of differentiation can be further expanded, enhancing their flexibility as a model system.

Stem Cell Services FAQs

What kind of cells can be cultured, characterized or differentiated?

We work with all kind of human iPS cell lines (healthy, diseased, modified). Other stem cell types can be analyzed or differentiated on demand.

Is it possible to purchase iPSC lines or iPSC-derived progeny from trenzyme?

Although we do have internal iPSC lines and validated differentiation protocols, we do not sell these iPSC lines or iPSC-derived progeny.

Does trenzyme have validated internal differentiation protocols, which can be used to differentiated customer iPSC lines towards a special cell type?

Yes, we do have internal differentiation protocols (e.g. neuronal, or hepatocyte protocols), which can be used in order to provide customized iPSC-derived cells. You will receive the generated and cryopreserved cells, as well as a comprehensive data sheet, however, no details regarding the differentiation protocol will be given.

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Tanja Waldmann, PhD Department Head Advanced Cell Systems_Scientific Expert For Your Cell Culture Project

Tanja Waldmann, PhD

Department Head Advanced Cell Systems

We would be happy to provide you with support on your cell culture research project. Contact us and let us know your questions and requests to our cell culture services. Our scientific experts will reply shortly.

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