Stem Cell Differentiation Service
Differentiation of human induced Pluripotent Stem Cells (hiPSC)
hiPSCs can be routinely generated from somatic cells of healthy donors or even patient-specific disease samples. After differentiation into tissue-specific cell types like neurons, cardiomyocytes or hepatocytes, hiPSCs can serve as next generation disease models in-a-dish, facilitating many applications in basic research, drug discovery and preclinical development.
Up to now, many applications in academia, biotech and pharmaceutical industry still rely on immortalized cancer cell lines as model system with all the drawbacks like genetic instability, aberrant gene expression patterns and dysfunctional signal pathways. Even the use of human primary cells has severe disadvantages like ethical problems, donor-to-donor variability and other general limitations (e.g. availability, limited expansion).
However, hiPSCs do not exhibit these limitations, have an unlimited expansion potential and can be differentiated into all cell types of the three germ layer lineages (ectoderm, mesoderm, endoderm). Moreover, intermediate differentiation stages can be further amplified.
Examples
For trenzyme’s Inhouse-Differentiation-Capabilities
Ectoderm
Neuronal differentiation
Characterization - IF staining
Mesoderm
Cardiomyocyte differentiation
Characterization - FACS analysis, Tox analysis & In vitro tox assay
Endoderm
Hepatocyte differentiation
Characterization - RT-PCR. ELISA
Run-Through Stem Cell Differentiation Service
If you have a proven hiPS cell line and an established differentiation protocol including validated QC markers, we can act as your time and cost saving extended workbench and provide you with the differentiated cells ready for use for your assays.
Furthermore, together with our clients, trenzyme is developing and optimizing new protocols for iPSC differentiation into cells of various lineages according to your specific project requirements.
Our expert service includes:
Customized Differentiation Service
If you do not have an established differentiation protocol and may also need to source the hiPS cell line as starting point, our experts will be able to support you with a milestone based R&D project.
We have extensive knowledge in development of protocols or may have the required protocol readily available. With access to a huge network of partners and technologies, we can ensure the progress of such a custom R&D project generating your desired cell type.
Our highly modular expert service includes:
What Our Clients Say About Us
“(…) cooperate with trenzyme for many years and even to intensify in the area of demanding iPS cell line projects.”
Dr. M. Bauer & Dr. R. Heilker, Boehringer Ingelheim Pharma GmbH & Co. KG
Your Benefits
From Working With Us
Transparency & Communication
Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.
Longstanding Expertise
With the extensive knowledge of our highly qualified scientific experts and with access to a huge network of partners and technologies, we complete your project successfully and efficiently.
Groundbreaking Technology
Since iPSC can be derived from healthy donors and disease patients, they serve as ideal systems for modelling disease phenotypes needed for drug screening and pharmaceutical development.
High Quality Standards
As an ISO-certified company working with a LIMS based documentation, all projects are completed following highest standards.
Relevant Publications Suitable to Our Service
Relevant Publications Suitable to Our Service
Our Stem Cell Experts
Department of Advanced Cell SystemsWe would be happy to provide you with support on your cell culture research project. Contact us and let us know your questions and requests to our cell culture services. Our scientific experts will reply shortly.