Stem Cell Characterization Service

Reprogramming human induced Pluripotent Stem Cells (hiPSCs) is a fundamental task of stem cell research. In order to provide reliable cell models for drug discovery and preclinical research, an extensive characterization of the generated hiPSC is essential. Our scientific team supports you with a comprehensive stem cell characterization service, investigates your hiPSC lines and provides you with reliability for your research project. We offer state-of-the-art characterization such as the directed trilineage differentiation for the proof of functional pluripotency in addition to the analysis of marker gene expression by RT-qPCR, immunostaining or flow cytometer.

In addition to the hiPSC characterization, trenzyme also offers a customized Stem Cell Differentiation Service and our Cell Line Expansion Service for banking of your hiPSC.

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Stem Cell Characterization Service

Characterization of human induced Pluripotent Stem Cells (hiPSC)

The field of human induced pluripotent stem cell (hiPSC) technology is a promising tool for drug discovery, toxicological in-vitro tests and regenerative medicine.

Since the first generation of hiPSCs, numerous reprogramming techniques have been established. However, the in-vitro process of reprogramming is still not fully understood. Often, the cellular stress, induced by the reprogramming process causes genetic and epigenetic alterations that can change the cellular phenotype and affect the integrity of experiments using these cell lines. It is therefore crucial to establish reliable molecular and functional assays to assess pluripotency and to exclude genetic aberrations due to the reprogramming process.

trenzyme’s routinely performed assays to validate your hiPSC lines include the characterization of cells for their expression of pluripotency markers via immunocytochemistry (ICC) and qPCR, molecular karyotyping, as well as the evaluation of their differentiation potential by directed trilineage differentiation.

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Process of human induced Pluripotent Stem Cell Characterization Service

Fully Characterized and Ready-To-Use hiPSC Master and Working Stocks

Process Steps of trenzyme's Stem Cell Characterization Service

What Our Clients Say About Us

trenzyme is one of our preferred service providers for cell line development and molecular biology services. The impressive level of personal customer liaison and the scientific competence are decisive factors for us to cooperate with trenzyme for many years and even to intensify in the area of demanding iPS cell line projects.

Dr. Margit Bauer & Dr. Ralf Heilker Boehringer Ingelheim Pharma GmbH & Co KG, Biberach, Germany
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human induced Pluripotent Stem Cell Characterization

& Banking Service

If you are engaged in stem cell research and are reprogramming hiPSC lines or initiating experiments with an unknown hiPSC line, our scientific stem cell experts can provide you with fully characterized and ready-to-use hiPSC master and working stocks. In this way, trenzyme can serve as an extended workbench, offering both time and cost savings.

Our Expert Service Includes:

  • Standardization of hiPSC culture parameters
  • Molecular and functional characterization of hiPSC lines
  • Banking of hiPSC master- and working stocks
  • Comprehensive hiPSC line datasheet

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Your Benefits

From Working With Us

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Transparency & Communication

Our dedicated project management will guide your project through every step of the process and keep you constantly informed on the progress. Transparency and high quality service are our mission.

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Longstanding Expertise

With the extensive knowledge of our highly qualified scientific experts and with access to a huge network of partners and technologies, we complete your project successfully and efficiently.

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Optimized Performance

The culture of iPSC is very challenging especially for not so experienced persons. With trenzyme´s long-lasting experience we provide optimized culture condition for your iPSC line

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High Quality Standards

As an ISO-certified company working with a LIMS based documentation, all projects are completed following highest standards.

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Stem Cell Characterization FAQs

What general quality control is performed on the iPSC lines?

All iPSC lines are subjected to a standard set of quality control assays, investigating sterility, mycoplasma, viability, and morphology.

What kind of assays will be performed to functionally characterize the iPSC lines?

In order to characterize iPSC lines for the expression of pluripotency markers, immunoflourescence staining and/or flow cytometry for key pluripotency markers OCT4, SOX2, SSEA-4, TRA-1-60, and TRA-1-81 will be performed. Furthermore, the ability to form all three germ layers will be investigated via directed differentiation towards endo-, meso-, and ectoderm (immunoflourescence staining, RT-qPCR).

What kind of karyotyping will be performed?

In order to evaluate genomic stability, chromosomal abnormalities, and copy number variants, molecular karyotyping via Illumina BeadArray will be applied.

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antje fuhrmann application & sales manager trenzyme
Antje Fuhrmann, PhD
Application & Sales Manager

We would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.

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