Stable Cell Line Development Service Provides Highly Customized Solutions For All Cell Lines & Targets
Our qualified scientific team at PhD and postdoctoral level can work with any cell line that is commercially available, delivered from your lab or selected from our collection. Our experienced team is dedicated to delivering highly customized solutions to ensure the success of your project.
We can handle projects under biosafety level 1 and even 2 (BSL1 or 2). For expression of your gene of interest, you can choose between our proprietary, robust and industry-leading ExoIN technology, available for constitutive and ➥inducible expression, or you can simply provide an expression construct suitable for your cell line. We offer several non-viral and viral gene delivery systems for the most efficient gene transfer even into difficult cell lines. After successful development of a stable cell line, either as a polyclonal cell pool or a monoclonal cell line, we confirm and validate the functional expression of your target protein according to your requirements and the nature of the target protein.
Our project management team, which is highly valued by our customers, will lead you through the whole project and keep you constantly informed on the project progress. That ensures highest flexibility, efficiency and will lead to maximum success.
Stable Cell Line Development Service Process
Why trenzyme is Your Preferred Service Provider for Stable Cell Line Development
Any Cell Line and Target
We can work with any commercially available cell line, our in-house available cell lines or with any cell line from your lab. Cell lines up to BSL2, iPSC or other difficult-to-handle cells are welcome.
Variety of Delivery Systems
We have optimized the processes and focused on chemical and physical gene delivery systems since they give excellent results also for difficult-to-transfect cell lines. But, if desired, we can also apply viral gene delivery systems to ensure that we generate your stable cell line based on any parental cell line.
Highly Customized Service
Whether you require a simple polyclonal fluorescent reporter cell line or a complex project involving the generation of a stable inducible cell line with coexpression of multiple targets, our unique ExoIN- or ExoINDUCE-based technology ensures industry-leading quality at challenging timelines.
One-stop-shop for Stable Cells
From sourcing the parental cells or selecting the appropriate in-house cell lines to generating a stable cell line and producing cell banks – we can take care of everything for you.
Free Up Your Resources
We are prepared to setup and optimize your new cell-based assay and deliver a 200-vial master cell bank with functional validation. In all cases, we ensure that the project meets your requirements and fits within your budget.
No Hidden Costs
No royalties, no license fees for R&D applications, no limitations. We only charge for the service we provide and commercial usage can be granted easily, if desired.
Extensive Experience with Various Cell Lines and Target Classes
Cell Lines
Human Cell Lines
- HEK293 (HEK-293; HEK/293; HEK 293; HEK,293; 293; 293 HEK; 293 Ad5; Human Embryonic Kidney 293)
- HEK293T (Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo)
- T-REx-293 (T-REx 293; T-REx293; TRex-293; TREx293; T-REx-HEK293; T-REx HEK 293; HEK293-T-REx; HEK293-Trex)
- Flp-In-T-REx-293 (Flp-In-T-REx 293; Flp-In T-REx-293; Flp-In T-REx 293; Flp-In T-Rex HEK293; Flp-In 293 T-Rex; Flp-In-T-REx; 293-Flip-in-TRex; HEK293 Flp-In T-REx; HEK293FlpIN; 293FlpIn T-Rex)
- HT1080 (Ht-1080; HT 1080; HT 1080.T)
- Jurkat (JM; JM-Jurkat; Jurkat-FHCRC; Jurkat FHCRC; FHCRC-11; FHCRC-Subklon 11; FCCH1024)
- Jurkat E6-1 (JurkatE6-1; Jurkat, Clone E6-1; Jurkat, Klon E6-1; Jurkat-Clone E6-1; Jurkat (Clone E6-1); Jurkat-Klon E6-1; Jurkat (Klon E6-1); JURKAT E-6.1; JURKAT E-61; Jurkat-E6; Jurkat E6; J.E6-1; E6-1; Jurkat J6)
- NK92 (NK-92; Natural Killer-92; NK-92.05; Neukoplast; aNK>)
- MCF-7 (MCF 7; MCF.7; MCF7; Michigan Cancer Foundation-7; ssMCF-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7)
- RPE-1 (hTERT RPE1; hTERT-RPE-1; hTERT RPE-1; hTERT-RPE; TERT-RPE1; RPE-1; RPE1; RPE1-hTERT; RPE1-hTert; RPE-hTERT)
- Raji (RAJI; P1-Raji)
- MDAH-041 (MDAH 041; 041S; MDAH041)
- K562 (K-562; K.562; K 562; KO; GM05372; GM05372E)
- THP-1 (THP1; THP 1; THPI; THP-1(ATCC); THP-1-O; O-THP-1; Tohoku Hospital Pediatrics-1)
- human iPSC lines (Human iPS Cell Line; Human iPSC; Induced Pluripotent Stem Cells; iPS Cell; iPSC)
- SH-SY5Y (SH-Sy5y; SHSY5Y; SHSY-5Y; SK-SH-SY5Y; SY5Y)
- LUHMES (LUnd Human MESencephalic; luhmes)
- U2OS (U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T)
- HepG2 (HEP-G2; Hep G2; HEP G2; HepG2; HEPG2)
- NCI-H716 (NCI H716; H716; H-716; NCIH716)
- Huh-7 (HuH-7; HUH-7; HuH7; Huh7; HUH7; HUH7.0; JTC-39; Japanese Tissue Culture-39)
- LNCaP (LNCAP; LNCap; Ln-Cap; LNCaP-RPCI; Lymph Node Carcinoma of the prostate)
- Ramos (RAMOS; Ramos 1; RA 1; RA.1; Ra #1; Ra No. 1; Ramos(RA1); Ramos-RA1; Ramos (RA 1); Ramos (RA #1); Ramos (RA)
Murine Cell Lines
- A20 (A-20)
- NIH3T3 (NIH/3T3; NIH-3T3; NIH 3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3)
- NSC-34 (NSC 34; NSC34; Neuroblastoma x Spinal Cord-34)
- NSO (NSO; NS/0; NS/O; NS-0)
- C2C12 (C2c12; C2-C12; C12)
- 3T3-L1 (3T3 L1; 3T3L1; 3T3-L1 ad; NIH-3T3-L1; NIH3T3-L1)
- B16-F10 (B16/F10; B16 F10; B16F10; B16 melanoma F10)
- CT26.WT (CT-26 WT; CT26.wt; CT26WT)
- Renca (RenCa; RENCA; Renal Carcinoma)
Rat Cell Lines
- PC12 (PC-12; PC 12; PC12.1)
- Rat2 (RAT2; Rat-2; RAT-2; Rat 2)
- MRMT-1 (MRMT 1; MRMT1; MRMT)
Hamster Cell Lines
- CHO-K1 (CHO K1; CHOK1; CHO cell clone K1; GM15452)
- CHO-S (CHO-s; CHOS)
- CHO-G alpha 16
Insect Cells
- HighFive (BTI-TN-5B1-4; BTI-TN5B1-4; BTI-Tn5B14; BTI-Tn 5B1-4; Tn-5B1-4; Tn 5B1-4; Tn5 B1-4; Tn5B1-4; TN5B14; TnH5; High Five; High 5; High-5; High5; Hi-five; Hi-5; Hi5; Tn-5)
- Sf9 (SF9; sf9; SF-9; Sf-9; sf-9; Sf 9; Spodoptera frugiperda clone 9; Sf clone 9; IPLB-Sf-9AE; IPLB-SF-9AE; IPLB-SF-9; IPLB-Sf-9; IPLB-Sf9)
- Sf21 (SF21; Sf-21; SF-21; IPLB-SF-21-AE; IPLB-Sf21-AE; IPLB-SF21-AE; IPLB-SF 21AE; IPLB-Sf21AE; IPLB-SF21AE; IPLB-SF-21; IPLB-Sf-21; IPLB-SF 21; IPLB-Sf21; IPLB-SF21)
Target Classes
Transmembrane Receptors
- Thyrosine kinase receptor (receptor tyrosine kinase; receptor-tyrosine kinase; receptor protein-tyrosine kinase; receptor-type protein-tyrosine kinase; transmembrane receptor protein tyrosine kinase; tyrosine kinase growth factor receptor; tyrosine receptor kinase; RTK; TKR; TRK)
- Thyrosine phosphate receptor (receptor tyrosine phosphatase; protein tyrosine phosphatase receptor; receptor-type protein-tyrosine phosphatase; transmembrane receptor protein tyrosine phosphatase; R-PTP; RPTP; receptor-type PTP; receptor protein-tyrosine phosphatase)
- G protein coupled receptor (G protein-coupled receptor; seven-transmembrane domain receptor; 7TM receptor; heptahelical receptor; serpentine receptor; G protein-linked receptor; GPLR; GPCR; 7TMR)
- Integrin receptors (cell adhesion receptors; extracellular matrix receptors; RGD receptors; transmembrane heterodimeric receptors; cell-surface adhesion molecules; ECM-binding receptors; cell-matrix adhesion proteins)
Cell surface receptors
- Cell surface receptors
Channel & Transporter Proteins
- Potassium channel (Voltage-gated potassium channels; Kv channels; inwardly rectifying potassium channels; Kir channels; Calcium-activated potassium channels; KCa channels; two-pore domain potassium channels; K2P channels; tandem pore domain potassium channels)
- Sodium-dependent dopamine transporter (Dopamine transporter; DAT; DA transporter; DAT1; Solute carrier family 6 member 3; SLC6A3; dopamine transporter)
- Glucose transporter (GLUT; GluT; glucose transporter protein; facilitative glucose transporter; solute carrier family 2; SLC2A)
- Glutamate/ aspartate transporter (Excitatory amino acid transporter; EAAT; glutamate transporter; sodium-dependent glutamate transporter; solute carrier family 1 member; SLC1; neurotransmitter transporter)
Intracellular Proteins
- Protein kinase inhibitor
Secreted Proteins
- Antibodies (immunoglobulin; Ig; immune globulin; gamma globulin; antibody protein; immunoglobulin protein; Ig protein; IgG1; IgG2; IgG3; IgG4; IgG; IgA; IgD; IgM; IgE)
- Extracellular matrix proteins (ECM proteins; matrix proteins; extracellular proteins; intercellular matrix proteins)
- Cytokines (lymphokines; monokines; chemokines; interleukins; interferons; tumour necrosis factors)
Other Targets
- Gqi5 (chimeric Gq protein; promiscuous G protein; G protein with exchanged C-terminal amino acids)
- Tet repressor (TetR; TetR protein; Tetracycline repressor protein; Tet repressor protein; Tetracycline-responsive repressor; Tc-responsive repressor; Tetracycline-controlled transcriptional repressor)
- Various reporter proteins
Modules & Explanation of trenzyme’s ExoIN Technology
The ExoIN technology links the expression of the target protein to the expression of a selection marker using the ExoIN tag. The target protein, selection marker and ExoIN tag are transcribed together as a single mRNA transcript. The subsequent co-translational cleavage at the ribosome immediately after the ExoIN tag results in an unmodified and functional target protein. In addition, the target protein and selection marker are expressed in a 1:1 stoichiometric ratio. This makes the ExoIN technology superior to conventional linker sequences such as IRES sequences, which often have variable stoichiometric expression ratios, or 2A peptides, which leave amino acid residues on the expressed proteins. The ExoIN technology offers you a highly flexible platform from single protein expression to co-expression of multiple proteins, where your target protein(s) can be combined with different promoters and selection markers to find the expression cassette and ultimately the combination that will give optimal expression levels in your final stable cell line.
This means that once selected, the stable cell pool will transcribe the target protein almost homogeneously. This unique technology allows easy and efficient selection of high quality stable cell populations with no risk of subsequent silencing or loss of expression, often eliminating the need for time-consuming and expensive single cell cloning for many applications.
Main Advantages of trenzyme’s ExoIN Technology
Watch Our ExoIN technology Video
Quality Control Assays
To ensure that you receive the highest quality and validity of your final cell lines, we carry out extensive quality control for our cell line development services. We confirm and validate the functional expression of your target protein according to your requirements and the nature of the target protein. Therefore, we are using a wide range of reliable and established QC methods, some of which are standard in our projects and some of which are optional. Just let us know your preferred method of analysis and we will apply it.
Included Analysis:
Optional Analysis:
Further Options of Stable Cell Line Development Service
Highly flexible and tailor-made solutions for your application. If you require an option that is not listed, please contact us – we will find the ideal solution for you:
What Our Clients Say About Us
“We were astonished by trenzyme’s recently delivered custom ExoIN cell lines (…)”
PD Dr. D. Wicher, Max Planck Institute for Chemical Ecology
Relevant Publications Suitable to Our Service
Relevant Publications Suitable to Our Service
Antje Fuhrmann, PhD
Application & Sales ManagerWe would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.