Stable Cell Line Development Service
Highly Customized Solutions
Our qualified scientific team at PhD and postdoctoral level can work with any cell line that is commercially available, delivered from your lab or selected from our collection. Our experienced team is dedicated to delivering highly customized solutions to ensure the success of your project. We can handle projects under biosafety level 1 and even 2 (BSL1 or 2). For expression of your gene of interest, you can choose between our proprietary, robust and industry-leading ExoIN technology, available for constitutive and ➥inducible expression, or you can simply provide an expression construct suitable for your cell line. We offer several non-viral and viral gene delivery systems for the most efficient gene transfer even into difficult cell lines. After successful development of a stable cell line, either as a polyclonal cell pool or a monoclonal cell line, we confirm and validate the functional expression of your target protein according to your requirements and the nature of the target protein.
Our project management team, which is highly valued by our customers, will lead you through the whole project and keep you constantly informed on the project progress. That ensures highest flexibility, efficiency and will lead to maximum success.
Process of
Stable Cell Line Development Service
Why trenzyme is Your Service Provider Of Choice
For Stable Cell Line Development
Any Cell Line and Target
We can work with any commercially available cell line, our in-house available cell lines or with any cell line from your lab. Cell lines up to BSL2, iPSC or other difficult-to-handle cells are welcome.
Variety of Delivery Systems
We have optimized the processes and focused on chemical and physical gene delivery systems since they give excellent results also for difficult-to-transfect cell lines. But, if desired, we can also apply viral gene delivery systems to ensure that we generate your stable cell line based on any parental cell line.
Highly Customized Service
Whether you require a simple polyclonal fluorescent reporter cell line or a complex project involving the generation of a stable inducible cell line with coexpression of multiple targets, our unique ExoIN- or ExoINDUCE-based technology ensures industry-leading quality at challenging timelines.
One-stop-shop for Stable Cells
From sourcing the parental cells or selecting the appropriate in-house cell lines to generating a stable cell line and producing cell banks – we can take care of everything for you.
Free Up Your Resources
We are prepared to setup and optimize your new cell-based assay and deliver a 200-vial master cell bank with functional validation. In all cases, we ensure that the project meets your requirements and fits within your budget.
No Hidden Costs
No royalties, no license fees for R&D applications, no limitations. We only charge for the service we provide and commercial usage can be granted easily, if desired.
Broad Experience
With Cell Lines and Target Classes
Cell Lines
Human Cell Lines
- HEK293
- HT1080
- Jurkat
- NK92
- MCF-7
- RPE-1
- Raji
- MDAH-041
- K562
- THP-1
- human iPSC lines
- SH-SY5Y
- LUHMES
- U2OS
- HepG2
- NCI-H716
- Huh-7
- LnCAP
- Ramos
Murine Cell Lines
- A20
- NIH3T3
- NSC-34
- NSO
- C2C12
- 3T3-L1
- B16-F10
- WT
- Renca
Rat Cell Lines
- PC12
- Rat2
- MRMT-1
Hamster Cell Lines
- CHO-K1
- CHO-S
- CHO-G alpha 16
Insect Cells
- HighFive
- Sf9
- Sf21
Target Classes
Transmembrane Receptors
- Thyrosine kinase receptor
- Thyrosine phosphate receptor
- G protein coupled receptor
- Integrin receptors
Cell surface receptors
- Cell surface receptors
Channel & Transporter Proteins
- Potassium channel
- Sodium-dependent dopamine transporter
- Glucose transporter
- Glutamate/ aspartate transporter
Intracellular Proteins
- Protein kinase inhibitor
Secreted Proteins
- Antibodies
- Extracellular matrix proteins
- Cyotokines
Other Targets
- Gqi5
- Tet repressor
- Various reporter proteins
trenzyme’s ExoIN Technology
Modules & Explanation
The ExoIN technology links the expression of the target protein to the expression of a selection marker using the ExoIN tag. The target protein, selection marker and ExoIN tag are transcribed together as a single mRNA transcript. The subsequent co-translational cleavage at the ribosome immediately after the ExoIN tag results in an unmodified and functional target protein. In addition, the target protein and selection marker are expressed in a 1:1 stoichiometric ratio. This makes the ExoIN technology superior to conventional linker sequences such as IRES sequences, which often have variable stoichiometric expression ratios, or 2A peptides, which leave amino acid residues on the expressed proteins. The ExoIN technology offers you a highly flexible platform from single protein expression to co-expression of multiple proteins, where your target protein(s) can be combined with different promoters and selection markers to find the expression cassette and ultimately the combination that will give optimal expression levels in your final stable cell line.
This means that once selected, the stable cell pool will transcribe the target protein almost homogeneously. This unique technology allows easy and efficient selection of high quality stable cell populations with no risk of subsequent silencing or loss of expression, often eliminating the need for time-consuming and expensive single cell cloning for many applications.
Main Advantages
of trenzyme’s ExoIN Technology
Watch our video
Quality Control Assays
To ensure that you receive the highest quality and validity of your final cell lines, we carry out extensive quality control for our cell line development services. We confirm and validate the functional expression of your target protein according to your requirements and the nature of the target protein. Therefore, we are using a wide range of reliable and established QC methods, some of which are standard in our projects and some of which are optional. Just let us know your preferred method of analysis and we will apply it.
Included Analysis:
Optional Analysis:
Further Options Stable Cell Line Development
Highly flexible and tailor-made solutions for your application. If you require an option that is not listed, please contact us – we will find the ideal solution for you:
What Our Clients Say About Us
We were astonished by trenzyme’s recently delivered custom ExoIN cell lines. Even “difficult-to-express” constructs show stable and robust expression. I highly recommend trenzyme’s cell line development service.
Max Planck Institute for Chemical Ecology, Jena, Germany
Relevant Publications of trenzyme’s Highly Qualified Scientific Team
Relevant Publications of trenzyme’s Highly Qualified Scientific Team
A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology. Int. J. Cosmet. Sci. 2022; 44: 216– 231. (2022)
Welzel J, Grüdl S, Banowski B, Stark H, Sättler A, and Welss T
Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine (2021)
Goyal P, Venkatraman Pai H, Kodali P, Vats B, Vajpai N, Annegowda S, Mane K, Mohan S, Saxena S, Bangalore Veerabhadraia A, Palande M, Sasankan Nair P, Chandrashekar More D, Rao Karudumpa U, Jyothirmai K, Bhattacharya A, Almeida F, Gulab Khyade S, Gouda S, Ranayhossaini DJ, Reddy Moole P, Smith JP, Barve A, Melarkode R, and Ullanat R
Development and validation of a cell based assay for the detection of neutralizing antibodies against recombinant insulins. J. Immunol. Methods 452, 53–62. (2018)
Chatterjee S, Vashishta L, Waichale VS, Nayak VG, Melarkode R, Donnelly CM, Vallano PT, Chirmule N, and Sengupta N
A botulinum toxin–derived targeted secretion inhibitor downregulates the GH/IGF1 axis. J. Clin. Invest. 122, 3295–3306. (2012)
Somm E, Bonnet N, Martinez A, Marks PMH, Cadd VA, Elliott M, Toulotte A, Ferrari SL, Rizzoli R, Hüppi PS, et al.
Toll-like receptor 4 variant D299G induces features of neoplastic progression in Caco-2 intestinal cells and is associated with advanced human colon cancer. Gastroenterology 141, 2154–2165. (2011)
Eyking A, Ey B, Rünzi M, Roig AI, Reis H, Schmid KW, Gerken G, Podolsky DK, and Cario E
Relevant Publications of trenzyme’s Highly Qualified Scientific Team
See all our relevant publications for Stable Cell Line Development Service
A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology. Int. J. Cosmet. Sci. 2022; 44: 216– 231. (2022)
Welzel J, Grüdl S, Banowski B, Stark H, Sättler A, and Welss T
Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine (2021)
Goyal P, Venkatraman Pai H, Kodali P, Vats B, Vajpai N, Annegowda S, Mane K, Mohan S, Saxena S, Bangalore Veerabhadraia A, Palande M, Sasankan Nair P, Chandrashekar More D, Rao Karudumpa U, Jyothirmai K, Bhattacharya A, Almeida F, Gulab Khyade S, Gouda S, Ranayhossaini DJ, Reddy Moole P, Smith JP, Barve A, Melarkode R, and Ullanat R
Development and validation of a cell based assay for the detection of neutralizing antibodies against recombinant insulins. J. Immunol. Methods 452, 53–62. (2018)
Chatterjee S, Vashishta L, Waichale VS, Nayak VG, Melarkode R, Donnelly CM, Vallano PT, Chirmule N, and Sengupta N
A botulinum toxin–derived targeted secretion inhibitor downregulates the GH/IGF1 axis. J. Clin. Invest. 122, 3295–3306. (2012)
Somm E, Bonnet N, Martinez A, Marks PMH, Cadd VA, Elliott M, Toulotte A, Ferrari SL, Rizzoli R, Hüppi PS, et al.
Toll-like receptor 4 variant D299G induces features of neoplastic progression in Caco-2 intestinal cells and is associated with advanced human colon cancer. Gastroenterology 141, 2154–2165. (2011)
Eyking A, Ey B, Rünzi M, Roig AI, Reis H, Schmid KW, Gerken G, Podolsky DK, and Cario E
Antje Fuhrmann, PhD
Application & Sales ManagerWe would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.