Inducible Expression Cell Line Service
based on unique ExoINDUCE technology
Our smart selection strategy enables highly effective inducible target protein expression in every mammalian cell line. Our ExoINDUCE system is an “All-in-one” system and one single plasmid encodes all features that are necessary for cell line generation: your target protein, the Tet/Dox-responsive transcriptional regulator and the selection marker.
➥trenzyme’s proprietary ExoIN technology couples the expression of the transcriptional activator to the expression of the selection marker at the protein level. This results in a streamlined selection process based on only one selection marker and only one transfection step. Due to this linkage, the risk of silencing or loss of the regulator is marginal. Despite that, we designed the ExoINDUCE system so that a second selection marker or reporter gene can be included, if desired.
Of course, you can also provide your own preferred expression construct suitable for the generation of an inducible cell line. As for the generation of a constitutively expressing cell line, we offer the same broad range of gene delivery systems (viral and non-viral) for the generation of your inducible expression cell line.
Process of Inducible Expression Cell Line Development
Highly Effective Inducible Target Protein Expression
Inducible Expression Cell Line Development
Highly Customized Service
After transfection/transduction and selection, we thoroughly characterize the resulting stable polyclonal cell pool or monoclonal cell line for inducibility and expression of your target protein using reliable standard methods, such as flow cytometry, Western Blotting, ELISA, IF or other functional readouts (contact us and simply name your preferred method). The use of the Tet/Dox-responsive transcriptional activator in the ExoINDUCE system leads to very low background expression levels and directly results in functional polyclonal cell pools after selection without clonal selection being a must (see Figure 1). In addition, generation of monoclonal cell lines allows for the selection of cell lines with different target expression and induction levels and even lower background expression (see Figure 2). Finally, we cryopreserve a quality-controlled research cell bank of your characterized and validated inducible cell line.
Figure 1: HEK293 parental and HEK293 ExoINDUCE-GFP pool.
HEK293 cells were transfected with ExoINDUCE all-in-one plasmid encoding GFP as target. A bulk pool was selected with hygromycin and analyzed 2 weeks after start of selection. For analysis, the bulk pool was induced by cultivation for 24h in medium containing 1 µg/mL doxycycline. 45.7 % GFP positive cells were detected by flow cytometry after induction.
Figure 2: HEK293 ExoINDUCE-GFP clones.
HEK293 ExoINDUCE-GFP clones were generated by limiting dilution of the bulk pool. Clones were analyzed 2 weeks after seeding and clones with different expression patterns were generated. For analysis, clones were induced by cultivation for 24h in medium containing 1 µg/mL doxycycline.
High, medium and low expressing clones are shown in histograms with and without doxycycline.
Main Advantages
of trenzyme’s ExoINDUCE Technology
What Our Clients Say About Us
We were astonished by trenzyme’s recently delivered custom ExoIN cell lines. Even “difficult-to-express” constructs show stable and robust expression. I highly recommend trenzyme’s cell line development service.
Max Planck Institute for Chemical Ecology, Jena, Germany
Antje Fuhrmann, PhD
Application & Sales ManagerWe would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.