Inducible Expression Cell Line Development Service

Inducible expression is a valuable tool for the expression of difficult-to-express and toxic target proteins and for the regulation of target expression. Our ExoINDUCE system combines the benefits of our industry-leading ExoIN technology with the proven Tet/Dox inducible expression system. This combination takes the Tet/Dox induction system to a new level of quality, enabling the ultra-fast generation of an inducible expression cell line that exhibits tight regulation and high levels of expression upon induction.

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Inducible Expression Cell Line Service

based on unique ExoINDUCE technology

Our smart selection strategy enables highly effective inducible target protein expression in every mammalian cell line. Our ExoINDUCE system is an “All-in-one” system and one single plasmid encodes all features that are necessary for cell line generation: your target protein, the Tet/Dox-responsive transcriptional regulator and the selection marker.

trenzyme’s proprietary ExoIN technology couples the expression of the transcriptional activator to the expression of the selection marker at the protein level. This results in a streamlined selection process based on only one selection marker and only one transfection step. Due to this linkage, the risk of silencing or loss of the regulator is marginal. Despite that, we designed the ExoINDUCE system so that a second selection marker or reporter gene can be included, if desired.

Of course, you can also provide your own preferred expression construct suitable for the generation of an inducible cell line. As for the generation of a constitutively expressing cell line, we offer the same broad range of gene delivery systems (viral and non-viral) for the generation of your inducible expression cell line.

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Process of Inducible Expression Cell Line Development

Highly Effective Inducible Target Protein Expression

Process of Inducible Expression Cell Line Development

Inducible Expression Cell Line Development

Highly Customized Service

After transfection/transduction and selection, we thoroughly characterize the resulting stable polyclonal cell pool or monoclonal cell line for inducibility and expression of your target protein using reliable standard methods, such as flow cytometry, Western Blotting, ELISA, IF or other functional readouts (contact us and simply name your preferred method). The use of the Tet/Dox-responsive transcriptional activator in the ExoINDUCE system leads to very low background expression levels and directly results in functional polyclonal cell pools after selection without clonal selection being a must (see Figure 1). In addition, generation of monoclonal cell lines allows for the selection of cell lines with different target expression and induction levels and even lower background expression (see Figure 2). Finally, we cryopreserve a quality-controlled research cell bank of your characterized and validated inducible cell line.

Figure 1: HEK293 parental and HEK293 ExoINDUCE-GFP pool.

HEK293 cells were transfected with ExoINDUCE all-in-one plasmid encoding GFP as target. A bulk pool was selected with hygromycin and analyzed 2 weeks after start of selection. For analysis, the bulk pool was induced by cultivation for 24h in medium containing 1 µg/mL doxycycline. 45.7 % GFP positive cells were detected by flow cytometry after induction.

trenzyme HEK293 ExoINDUCE GFP cell pools
trenzyme HEK293 ExoINDUCE GFP clones

Figure 2: HEK293 ExoINDUCE-GFP clones.

HEK293 ExoINDUCE-GFP clones were generated by limiting dilution of the bulk pool. Clones were analyzed 2 weeks after seeding and clones with different expression patterns were generated. For analysis, clones were induced by cultivation for 24h in medium containing 1 µg/mL doxycycline.
High, medium and low expressing clones are shown in histograms with and without doxycycline.

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Main Advantages

of trenzyme’s ExoINDUCE Technology

  • Save time and money with our “all-in-one system” – only one round of cell line generation and clone screening is required to obtain the final inducible cell line
  • Continuous selection of regulator expression on protein level (no silencing / no loss of regulator possible)
  • Beneficial when selecting sensitive cell lines as only one antibiotic is needed for selection
  • No license fee and no royalties are due for generation and further use of your stable inducible cell line*
  • ExoINDUCE technology is applicable to any cell line and all target classes
  • Generation of ready-to-use stable assay cell lines within weeks – depending on target and parental cell line no single cell cloning is needed
  • Clones with different target expression levels can be selected upon limiting dilution of the parental ExoINDUCE cell pool
  • To fully comply with your requirements and the quantity of cells you need, we offer further options with our cell expansion and cell banking service.
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What Our Clients Say About Us

We were astonished by trenzyme’s recently delivered custom ExoIN cell lines. Even “difficult-to-express” constructs show stable and robust expression. I highly recommend trenzyme’s cell line development service.

PD Dr. D. Wicher Max Planck Institute for Chemical Ecology, Jena, Germany
clients logo max planck institute for chemical ecology trenzyme
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Inducible Expression Cell Line FAQs

What is the benefit of the ExoINDUCE system in comparison to other inducible systems?

The ExoINDUCE system works as an “one plasmid system” and all elements for the generation of an inducible cell line are located on one plasmid. Due to the elaborated design of the system and the proven selection strategy, there is no need to generate a regulator cell line first – the final inducible cell line can be generated in only one step saving a lot of time and money.

Which target classes have you already worked with?

We already worked with all different target classes: transmembrane receptors, channels & transporters, intracelllular proteins, secreted proteins and many other target classes.

Does trenzyme only offer the generation of stable, inducible cell lines using the ExoINDUCE system?

No, if you provide your preferred parental cell line and your inducible expression system of choice, we will generate the recombinant cell line according to your needs.

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antje fuhrmann application & sales manager trenzyme
Antje Fuhrmann, PhD
Application & Sales Manager

We would be happy to provide you with support on your cell research project. Contact us and let us know your questions and requests to our cell services. Our scientific experts will reply shortly.

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