Cell Line Adaption Service

Our cell line adaption service takes your cells and adapts them to your desired level of serum-free medium, from chemically defined media to animal component-free media, even to GMP compliant levels. This service is of value to all laboratories requiring defined and reproducible conditions, such as toxicological and pharmacological testing laboratories. In addition, the EU and US authorities are promoting toxicological and pharmacological testing in accordance with the 3Rs, which is also a strong argument for the elimination of FBS.

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Cell Line Adaption Service

Full Service to Adapt Your Cells to Serum-Free Conditions

For decades, standard cell culture has been performed in the presence of fetal bovine serum (FBS), which contains growth factors required for cell growth. Basically, FBS is a by-product of cattle farming (meat industry) and its quality depends strongly on the health of the animals and their conditions during this process (general health, nutrition, stress, etc.). Apart from the ethical issues of avoiding the use of animals in science and industry, this fact massively increases the variability of cultures supplemented with FBS, presents a reproducibility problem and is one of the reasons for the reproducibility crisis in science.

Our cell culture experts offer a complete service to adapt your cells to serum-free conditions by our cell line adaptation service. Depending on your requirements, we can adapt your cells to xeno-free, chemically defined (CD) or animal derived component free (ADCF) media. We also offer adaptation as a GMP-compliant service (GMP Services) and provide you with a cell bank by our cell expansion service. In addition, these cells can be used directly in our cell-based assay service in which we use your adapted cells and perfom your assay in-house and the resulting data will be provided to you (cell-based assay service). You will receive full information on the supplements and their concentrations together with a comprehensive data sheet for your new cell line.

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Cell Line Adaption Service

Main Advantages

Cell Line Adaption Service_FBS Free Medium

FBS Free Cell Line According to the 3R Rules

Cell line adaption to the princible of the 3Rs (Replacement, Reduction and Refinement). The EU and US authorities are promoting toxicological and pharmaceutical testing according to the 3Rs, which is a strong argument for eliminating FBS from cell culture medium. With our cell line adaptation service, we take your cells and adapt them to your desired level of serum-free medium.

Service Available for Any Cell Line & Target

trenzyme's cell line adaption service is available for any cell line and target. Our highly qualified cell culture experts adapt your specific cell line to your desired level of serum-free medium. We can work with any commercially available cell line, our in-house available cell lines or with any cell line from your laboratory. Even cell lines up to BSL2, iPSC or other difficult-to-handle cells are also welcome.

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High Level of Expertise & Full Transparency of Media Composition

Our cell culture experts do not use pre-mixed media from any particular manufacturer. Instead, our approach for developing media is based upon our longstanding expertise regarding the supplementary requirements of a wide range of cell types and cell lines. To ensure transparency, we provide comprehensive information regarding the composition of the media and the recommended procedures for handling the new cell line.

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Process of

Cell Line Adaption Service

Process of Cell Line Adaption Service_trenzyme
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Cell Line Adaption Service  FAQs

How do you define ADCF (animal-derived component-free) cell culture?

ADCF media do not contain supplements derived from animal (including human) body fluids (e.g. FBS, serum albumin, etc.) and do not use other animal-derived materials commonly used in cell culture. This includes dissociation enzymes purified from animal tissues, such as trypsin, which is typically extracted from porcine pancreas.

How do you define Xeno-free cell culture?

Xeno-free media may contain primary human tissue material, such as human serum, but no components of animal origin.

How do you define chemically (CD) defined cell culture?

Chemically defined media (CD) may only contain supplements of known concentration and structure. This includes recombinant proteins such as cytokines or growth factors, but not components of animal (including human) origin.

Why should I use ADCF or CD media?

Media components are typically synthetic or derived from plants, microbes or recombinant technology. ADCF media are particularly important in the pharmaceutical and biotechnology industries because they reduce variability in experiments, minimise the risk of contamination (such as animal prions or viruses), and often address ethical concerns regarding the use of animal products.

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Tanja Waldmann, PhD Department Head Advanced Cell Systems_Scientific Expert For Your Cell Culture Project
Tanja Waldmann, PhD
Department Head Advanced Cell Systems

We would be happy to provide you with support on your cell culture research project. Contact us and let us know your questions and requests to our cell culture services. Our scientific experts will reply shortly.

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