Cell Line Adaption Service

Our cell line adaption service takes your cells and adapts them to your desired level of serum-free medium, from chemically defined media to animal component-free media, even to GMP compliant levels. This service is of value to all laboratories requiring defined and reproducible conditions, such as toxicological and pharmacological testing laboratories. In addition, the EU and US authorities are promoting toxicological and pharmacological testing in accordance with the 3Rs, which is also a strong argument for the elimination of FBS.

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Cell Line Adaption Service

Full Service to Adapt Your Cells to Serum-Free Conditions

For decades, standard cell culture has been performed in the presence of fetal bovine serum (FBS), which contains growth factors required for cell growth. Basically, FBS is a by-product of cattle farming (meat industry) and its quality depends strongly on the health of the animals and their conditions during this process (general health, nutrition, stress, etc.). Apart from the ethical issues of avoiding the use of animals in science and industry, this fact massively increases the variability of cultures supplemented with FBS, presents a reproducibility problem and is one of the reasons for the reproducibility crisis in science.

Our cell culture experts offer a complete service to adapt your cells to serum-free conditions by our cell line adaptation service. Depending on your requirements, we can adapt your cells to xeno-free, chemically defined (CD) or animal derived component free (ADCF) media. We also offer adaptation as a GMP-compliant service (GMP Services) and provide you with a cell bank by our cell expansion service. In addition, these cells can be used directly in our cell-based assay service in which we use your adapted cells and perfom your assay in-house and the resulting data will be provided to you (cell-based assay service). You will receive full information on the supplements and their concentrations together with a comprehensive data sheet for your new cell line.

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Cell Line Adaption Service

Main Advantages

Cell Line Adaption Service_FBS Free Medium

FBS Free Cell Line According to the 3R Rules

Cell line adaption to the principle of the 3Rs (Replacement, Reduction and Refinement). The EU and US authorities are promoting toxicological and pharmaceutical testing according to the 3Rs, which is a strong argument for eliminating FBS from cell culture medium. With our cell line adaptation service, we take your cells and adapt them to your desired level of serum-free medium.

Service Available for Any Cell Line & Target

trenzyme's cell line adaption service is available for any cell line and target. Our highly qualified cell culture experts adapt your specific cell line to your desired level of serum-free medium. We can work with any commercially available cell line, our in-house available cell lines or with any cell line from your laboratory. Even cell lines up to BSL2, iPSC or other difficult-to-handle cells are also welcome.

Cell Line Adaption Service_Medium Bottle

High Level of Expertise & Full Transparency of Media Composition

Our cell culture experts do not use pre-mixed media from any particular manufacturer. Instead, our approach for developing media is based upon our longstanding expertise regarding the supplementary requirements of a wide range of cell types and cell lines. To ensure transparency, we provide comprehensive information regarding the composition of the media and the recommended procedures for handling the new cell line.

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Process of

Cell Line Adaption Service

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Feasibility Check_Cell Line Adaption Service_trenzyme GmbH

Feasibility Check & Project Consultion

The feasibility study involves a close dialogue between the customer and our cell culture experts. The customer's requirements are discussed and finally our cell culture experts will suggest you one or more reasonable medium compositions.

Once the customized quotation has been prepared by our team and accepted by the customer, the project can begin.

QC of Incoming Cell Line_Cell Line Adaption Service_trenzyme GmbH

QC of Incoming Cell Line

During this step of the project we will confirm the culture conditions of the external cell line including mycoplasma test. This step is not necessary when a trenzyme in-house cell line will be used for the project.

  • Thawing and cultivation of cells
  • Thaw-viability assay, microscopic evaluation and mycoplasma test
  • Verification of culture conditions
  • Cryopreservation of a small cell bank

Cell Line Adaption

Depending on the cell line that should be adapted it can be beneficial to perform a stepwise adaption or to perform a so-called “do-or-die”-approach.

During adaption the growth of your cell line will be monitored and documented. You will receive full documentation and medium composition in a comprehensive data sheet or optionally a final report. Viability and growth rates will be monitored and documented over several passages for both approaches

  • Step-Wise Adaption: The old medium will be replaced slowly in several steps (e.g. 25%, 50%, 75%, 100%)
  • „Do-or-Die“-Approach: The old medium will be replaced in one step at once

 

Cell Line Expansion_Cell Line Adaption Service_trenzyme GmbH

Cell Line Expansion & Cryopreservation

The adapted cells will be maintained in culture for a few passages in order to validate successful adaption to the new medium. After validation we will expand the cells and cryopreserve them according to your needs (freezing media, number of cells / vial, number of vials).

  • Expansion of adapted cell line
  • Cryopreservation of a cell bank

Quality Control_Cell Line Adaption Service_trenzyme GmbH

Quality Control of the Adapted Cell Line

We thaw your newly adapted cryopreserved cell line to scrutinise viability of the cell line. In order to verify the culture conditions we will passage the cells for another three times.

Standard Quality Control: 

  • Thaw-Viability Assay
  • Mycoplasma Test for your security

Further Optional Analysis Methods According to Your Needs:

  • Flow Cytometry ( i.e. expression of specific proteins/genes)
  • Immunflourescence
  • PCR
  • Western Blot
  • ELISA
  • RT-qPCR

Shipment - Fast Delivery

  • Worldwide Shipping 
    We offer global shipping while adhering to embargo regulations, ensuring reliable delivery to your location.
  • Fast Delivery Times
    Our logistics team works with trusted partners to guarantee swift delivery without compromising product quality.
  • Reliable Logistics Network
    We collaborate with renowned delivery providers who operate efficient worldwide logistics systems.
  • Parcel Tracking for Transparency
    You’ll receive a tracking code upon dispatch, allowing continuous monitoring of your shipment's progress.

 

Deliverables_Cell Line Adaption Service_trenzyme GmbH

Deliverables

Together with your cryopreserved cells you will obtain comprehensive documentation including

  • Data sheet with exact culture conditions and media compositions
  • Growth rate documentation
  • Optional: a full report about the whole adaption process

 

Timeline_Cell Line Adaption Service_trenzyme GmbH

Timeline

  • The timeline very much depends on the cell line, the doubling rate of the cells and on the type of medium (low or high nutrient content) to which the cells shall be adapted. Therefore timeline ranges from 6 to 12 weeks.

 

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Application Note

Adaption of Cell Lines to Serum-Free Media

Highly Customized Cell Line Adaption Service_Titel_Application Note_Cell Line Adaption_trenzyme GmbH_20241129
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Cell Line Adaption Service  FAQs

How do you define ADCF (animal-derived component-free) cell culture?

ADCF media do not contain supplements derived from animal (including human) body fluids (e.g. FBS, serum albumin, etc.) and do not use other animal-derived materials commonly used in cell culture. This includes dissociation enzymes purified from animal tissues, such as trypsin, which is typically extracted from porcine pancreas.

How do you define Xeno-free cell culture?

Xeno-free media may contain primary human tissue material, such as human serum, but no components of animal origin.

How do you define chemically (CD) defined cell culture?

Chemically defined media (CD) may only contain supplements of known concentration and structure. This includes recombinant proteins such as cytokines or growth factors, but not components of animal (including human) origin.

Why should I use ADCF or CD media?

Media components are typically synthetic or derived from plants, microbes or recombinant technology. ADCF media are particularly important in the pharmaceutical and biotechnology industries because they reduce variability in experiments, minimise the risk of contamination (such as animal prions or viruses), and often address ethical concerns regarding the use of animal products.

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Tanja Waldmann, PhD Department Head Advanced Cell Systems_Scientific Expert For Your Cell Culture Project

Tanja Waldmann, PhD

Department Head Advanced Cell Systems

We would be happy to provide you with support on your cell culture research project. Contact us and let us know your questions and requests to our cell culture services. Our scientific experts will reply shortly.

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