Cell Based Assay Development Service

Our scientific experts have many years of experience in toxicological and pharmacological cell-based assay development. trenzyme offers the unique advantage that we can handle any cell type (cell lines, iPSC-derived cells, primary cells etc.). Just tell us your requirements (cell type, biological & technical endpoint) and our team will implement a comprehensive protocol, perform the assay in our laboratories and provide you with the desired data.

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Cell Based Assay Development Service – Save Valuable Time For Your Research Project

Current drug development and toxicological testing strategies can be costly and often have high failure rates in later stages of development. Accordingly, cell-based assays are important tools for screening new drug candidates before moving on to expensive animal model testing. Our experienced assay experts are dedicated to accelerating your screening capabilities. With our many years of experience in cell-based assay development, you can save valuable time for your research project by outsourcing time-consuming workflows to us.

Pharmaceutical companies use a variety of approaches to drug discovery. Historically, large numbers of compounds have been tested in high-throughput biochemical assays. However, the complexity of disease backgrounds has increased the need for more physiological systems and the use of cell-based assays to predict the response of the organism. trenzyme specialises in cell-based assays using various cell lines and state-of-the-art technologies. Our highly skilled experts are able to develop customized cell-based assays and provide reliable results to help you accelerate your screening capabilities. In addition, together with our stem cell experts, complex predictive and translational in vitro assays can be combined with highly physiological human stem cell-derived cell types to provide advanced compound testing solutions.

Cell Based Assay Development Service

Main Advantages

  • Benefit from a full technology transfer of your assay or let our assay service develop your assays according to your needs.
  • Development of assays based on basic cell lines, customized cell lines or iPSC-derived cell types.
  • Best starting point for your assay development, drug screening and functional studies
  • Broad expertise in assay development for in-vitro-toxicology and pharmaceutical testing.
  • Wide range of techniques to measure endpoints (FACS, Immunostaining, ELISA, Western blot, qPCR).
  • Our custom ➥ cell line development service enables the production of assay cell line and therefore the whole process of cell-based assay development is possible from one-stop.
  • GMP-like documentation available on request.

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Cell Based Assay Development Service

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Your Benefits From Working With Us

trenzyme’s cell-based assay services provide customized solutions for drug discovery, biologics characterization and quality control. We offer a wide range of in vitro cell-based assays, including flow cytometry assays, cell-based binding assays, tailored to your specific research needs. Our expertise ensures reliable and reproducible results, supporting the development of high-quality therapeutics.

With extensive experience in cell-based in vitro assays, we design and optimize cell-based assays for applications such as potency testing, mode-of-action studies, and biomarker analysis. Whether you need cell-based assay examples for early-stage research or validated assays for regulatory submission, trenzyme delivers high-performance solutions.

Let us help accelerate your drug development with our state-of-the-art cell-based assay development services!

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Transparency & Communication

Your project manager keeps you constantly informed on the progress of your project.

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Longstanding Expertise

Since 2000, our highly qualified scientific experts accelerate your innovation.

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Optimized Performance

trenzyme‘s assay service enable a time-saving workflow for your project.

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High Quality Standards

As an ISO-certified company, all projects are completed following high standards.

What Our Clients Say About Us

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“(…) we have carried out many different projects within trenzyme’s cell-based assay service. We particularly appreciate the fast and excellent communication, (…)”

Dr. K. Berger, breeding botanicals international AG

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Application Note

Cell Based Assay Development Services using a standard cell line: HepG2

Application Note_Cell Based Assay Development Service
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Relevant Publications Suitable to Our Service

Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test. Chemical Research in Toxicology 2022 35 (5), 760-773 (2022)

Cherianidou A, Seidel F, Kappenberg F, Dreser N, Blum J, Waldmann T, Blüthgen N, Meisig J, Madjar K, Henry M, Rotshteyn T, Marchan R, Edlund K, Leist M, Rahnenführer J, Sachinidis A, and Hengstler JG

 

➥Read the article

A human stem cell-derived test system for agents modifying neuronal N-methyl-D-aspartate-type glutamate receptor Ca2+-signalling. Arch Toxicol. 2021 May;95(5):1703-1722. (2021)

Klima S, Brüll M, Spreng AS, Suciu I, Falt T, Schwamborn JC, Waldmann T, Karreman C, Leist M

 

➥Read the article

Development of a neural rosette formation assay (RoFA) to identify neurodevelopmental toxicants and to characterize their transcriptome disturbances. Arch Toxicol. 2020 Jan;94(1):151-171. doi: 10.1007/s00204-019-02612-5. Epub 2019 Nov 11. PMID: 31712839. (2020)

Dreser N, Madjar K, Holzer AK, Kapitza M, Scholz C, Kranaster P, Gutbier S, Klima S, Kolb D, Dietz C, Trefzer T, Meisig J, van Thriel C, Henry M, Berthold MR, Blüthgen N, Sachinidis A, Rahnenführer J, Hengstler JG, Waldmann T, and Leist M

 

➥Read the article

Establishment of a human iPS cell-derived neuronal model cell to study synaptic signaling (2020)

Traub S

 

➥Read the article

The EU-ToxRisk method documentation, data processing and chemical testing pipeline for the regulatory use of new approach methods. Arch Toxicol. 2020 Jul;94(7):2435-2461. doi: 10.1007/s00204-020-02802-6. Epub 2020 Jul 6. PMID: 32632539; PMCID: PMC7367925. (2020)

Krebs A, van Vugt-Lussenburg BMA, Waldmann T, Albrecht W, Boei J, Ter Braak B, Brajnik M, Braunbeck T, Brecklinghaus T, Busquet F, Dinnyes A, Dokler J, Dolde X, Exner TE, Fisher C, Fluri D, Forsby A, Hengstler JG, Holzer AK, Janstova Z, Jennings P, Kisitu J, Kobolak J, Kumar M, Limonciel A, Lundqvist J, Mihalik B, Moritz W, Pallocca G, Ulloa APC, Pastor M, Rovida C, Sarkans U, Schimming JP, Schmidt BZ, Stöber R, Strassfeld T, van de Water B, Wilmes A, van der Burg B, Verfaillie CM, von Hellfeld R, Vrieling H, Vrijenhoek NG, and Leist M

 

➥Read the article

Examination of microcystin neurotoxicity using central and peripheral human neurons. ALTEX. 2021;38(1):73-81. doi: 10.14573/altex.2003182. Epub 2020 Jun 23. PMID: 32591837. (2020)

Klima S, Suciu I, Hoelting L, Gutbier S, Waldmann T, Dietrich DR, and Leist M

 

➥Read the article

Template for the description of cell-based toxicological test methods to allow evaluation and regulatory use of the data. ALTEX. 2019;36(4):682-699. doi: 10.14573/altex.1909271. Erratum in: ALTEX. 2020;37(1):164. PMID: 31658359. (2019)

Krebs A, Waldmann T, Wilks MF, Van Vugt-Lussenburg BMA, Van der Burg B, Terron A, Steger-Hartmann T, Ruegg J, Rovida C, Pedersen E, Pallocca G, Luijten M, Leite SB, Kustermann S, Kamp H, Hoeng J, Hewitt P, Herzler M, Hengstler JG, Heinonen T, Hartung T, Hardy B, Gantner F, Fritsche E, Fant K, Ezendam J, Exner T, Dunkern T, Dietrich DR, Coecke S, Busquet F, Braeuning A, Bondarenko O, Bennekou SH, Beilmann M, and Leist M

 

➥Read the article

Major changes of cell function and toxicant sensitivity in cultured cells undergoing mild, quasi-natural genetic drift. Arch Toxicol. 2018 Dec;92(12):3487-3503. doi: 10.1007/s00204-018-2326-5. Epub 2018 Oct 8. Erratum in: Arch Toxicol. 2019 Jun;93(6):1771. PMID: 30298209; PMCID: PMC6290691. (2018)

Gutbier S, May P, Berthelot S, Krishna A, Trefzer T, Behbehani M, Efremova L, Delp J, Gstraunthaler G, Waldmann T, Leist M

 

➥Read the article

Recommendation on test readiness criteria for new approach methods in toxicology: Exemplified for developmental neurotoxicity. ALTEX. 2018;35(3):306-352. doi: 10.14573/altex.1712081. Epub 2018 Feb 23. Erratum in: ALTEX. 2019;36(3):506. PMID: 29485663; PMCID: PMC6545888. (2018)

Bal-Price A, Hogberg HT, Crofton KM, Daneshian M, FitzGerald RE, Fritsche E, Heinonen T, Hougaard Bennekou S, Klima S, Piersma AH, Sachana M, Shafer TJ, Terron A, Monnet-Tschudi F, Viviani B, Waldmann T, Westerink RHS, Wilks MF, Witters H, Zurich MG, and Leist M

 

➥Read the article

Stage-specific metabolic features of differentiating neurons: Implications for toxicant sensitivity. Toxicol Appl Pharmacol. 2018 Sep 1;354:64-80. doi: 10.1016/j.taap.2017.12.013. Epub 2017 Dec 24. Erratum in: Toxicol Appl Pharmacol. 2019 Jun 1;372:70. PMID: 29278688. (2018)

Delp J, Gutbier S, Cerff M, Zasada C, Niedenführ S, Zhao L, Smirnova L, Hartung T, Borlinghaus H, Schreiber F, Bergemann J, Gätgens J, Beyss M, Azzouzi S, Waldmann T, Kempa S, Nöh K, and Leist M

 

➥Read the article

Adverse outcome pathways: opportunities, limitations and open questions. Arch Toxicol. 2017 Nov;91(11):3477-3505. doi: 10.1007/s00204-017-2045-3. Epub 2017 Oct 19. PMID: 29051992. (2017)

Leist M, Ghallab A, Graepel R, Marchan R, Hassan R, Bennekou SH, Limonciel A, Vinken M, Schildknecht S, Waldmann T, Danen E, van Ravenzwaay B, Kamp H, Gardner I, Godoy P, Bois FY, Braeuning A, Reif R, Oesch F, Drasdo D, Höhme S, Schwarz M, Hartung T, Braunbeck T, Beltman J, Vrieling H, Sanz F, Forsby A, Gadaleta D, Fisher C, Kelm J, Fluri D, Ecker G, Zdrazil B, Terron A, Jennings P, van der Burg B, Dooley S, Meijer AH, Willighagen E, Martens M, Evelo C, Mombelli E, Taboureau O, Mantovani A, Hardy B, Koch B, Escher S, van Thriel C, Cadenas C, Kroese D, van de Water B, and Hengstler JG

 

➥Read the article

Combination of multiple neural crest migration assays to identify environmental toxicants from a proof-of-concept chemical library. Arch Toxicol. 2017 Nov;91(11):3613-3632. doi: 10.1007/s00204-017-1977-y. Epub 2017 May 5. PMID: 28477266. (2017)

Nyffeler J, Dolde X, Krebs A, Pinto-Gil K, Pastor M, Behl M, Waldmann T, and Leist M

 

➥Read the article

Stem Cell-Derived Immature Human Dorsal Root Ganglia Neurons to Identify Peripheral Neurotoxicants. Stem Cells Transl Med. 2016 Apr;5(4):476-87. doi: 10.5966/sctm.2015-0108. Epub 2016 Mar 1. PMID: 26933043; PMCID: PMC4798731. (2016)

Hoelting L, Klima S, Karreman C, Grinberg M, Meisig J, Henry M, Rotshteyn T, Rahnenführer J, Blüthgen N, Sachinidis A, Waldmann T, and Leist M

 

➥Read the article

Identification of transcriptome signatures and biomarkers specific for potential developmental toxicants inhibiting human neural crest cell migration. Arch Toxicol. 2016 Jan;90(1):159-80. doi: 10.1007/s00204-015-1658-7. Epub 2015 Dec 26. PMID: 26705709; PMCID: PMC4710658. (2016)

Pallocca G, Grinberg M, Henry M, Frickey T, Hengstler JG, Waldmann T, Sachinidis A, Rahnenführer J, and Leist M

 

➥Read the article

A LUHMES 3D dopaminergic neuronal model for neurotoxicity testing allowing long-term exposure and cellular resilience analysis. Arch Toxicol. 2016 Nov;90(11):2725-2743. doi: 10.1007/s00204-015-1637-z. Epub 2015 Dec 8. PMID: 26647301; PMCID: PMC5065586. (2016)

Smirnova L, Harris G, Delp J, Valadares M, Pamies D, Hogberg HT, Waldmann T, Leist M, and Hartung T

 

➥Read the article

A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors. Arch Toxicol. 2015 Sep;89(9):1599-618. doi: 10.1007/s00204-015-1573-y. Epub 2015 Aug 14. PMID: 26272509; PMCID: PMC4551554. (2015)

Rempel E, Hoelting L, Waldmann T, Balmer NV, Schildknecht S, Grinberg M, Das Gaspar JA, Shinde V, Stöber R, Marchan R, van Thriel C, Liebing J, Meisig J, Blüthgen N, Sachinidis A, Rahnenführer J, Hengstler JG, and Leist M

 

➥Read the article

Grouping of histone deacetylase inhibitors and other toxicants disturbing neural crest migration by transcriptional profiling. Neurotoxicology. 2015 Sep;50:56-70. doi: 10.1016/j.neuro.2015.07.008. Epub 2015 Jul 31. PMID: 26238599. (2015)

Dreser N, Zimmer B, Dietz C, Sügis E, Pallocca G, Nyffeler J, Meisig J, Blüthgen N, Berthold MR, Waldmann T, and Leist M

 

➥Read the article

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation. J Vis Exp. 2015 Jun 17;(100):e52333. doi: 10.3791/52333. PMID: 26132533; PMCID: PMC4544843. (2015)

Shinde V, Klima S, Sureshkumar PS, Meganathan K, Jagtap S, Rempel E, Rahnenführer J, Hengstler JG, Waldmann T, Hescheler J, Leist M, and Sachinidis A

 

➥Read the article

Toxicogenomics directory of chemically exposed human hepatocytes. Arch Toxicol. 2014 Dec;88(12):2261-87. doi: 10.1007/s00204-014-1400-x. Epub 2014 Nov 16. PMID: 25399406. (2014)

Grinberg M, Stöber RM, Edlund K, Rempel E, Godoy P, Reif R, Widera A, Madjar K, Schmidt-Heck W, Marchan R, Sachinidis A, Spitkovsky D, Hescheler J, Carmo H, Arbo MD, van de Water B, Wink S, Vinken M, Rogiers V, Escher S, Hardy B, Mitic D, Myatt G, Waldmann T, Mardinoglu A, Damm G, Seehofer D, Nüssler A, Weiss TS, Oberemm A, Lampen A, Schaap MM, Luijten M, van Steeg H, Thasler WE, Kleinjans JC, Stierum RH, Leist M, Rahnenführer J, and Hengstler JG

 

➥Read the article

Profiling of drugs and environmental chemicals for functional impairment of neural crest migration in a novel stem cell-based test battery. Arch Toxicol. 2014 May;88(5):1109-26. doi: 10.1007/s00204-014-1231-9. Epub 2014 Apr 2. PMID: 24691702; PMCID: PMC3996367. (2014)

Zimmer B, Pallocca G, Dreser N, Foerster S, Waldmann T, Westerhout J, Julien S, Krause KH, van Thriel C, Hengstler JG, Sachinidis A, Bosgra S, and Leist M

 

➥Read the article

From transient transcriptome responses to disturbed neurodevelopment: role of histone acetylation and methylation as epigenetic switch between reversible and irreversible drug effects. Arch Toxicol. 2014 Jul;88(7):1451-68. doi: 10.1007/s00204-014-1279-6. Epub 2014 Jun 17. PMID: 24935251; PMCID: PMC4067541. (2014)

Balmer NV, Klima S, Rempel E, Ivanova VN, Kolde R, Weng MK, Meganathan K, Henry M, Sachinidis A, Berthold MR, Hengstler JG, Rahnenführer J, Waldmann T, and Leist M

 

➥Read the article

Relevant Publications Suitable to Our Service

Open to see relevant publications

Classification of Developmental Toxicants in a Human iPSC Transcriptomics-Based Test. Chemical Research in Toxicology 2022 35 (5), 760-773 (2022)

Cherianidou A, Seidel F, Kappenberg F, Dreser N, Blum J, Waldmann T, Blüthgen N, Meisig J, Madjar K, Henry M, Rotshteyn T, Marchan R, Edlund K, Leist M, Rahnenführer J, Sachinidis A, and Hengstler JG

➥Read the article

A human stem cell-derived test system for agents modifying neuronal N-methyl-D-aspartate-type glutamate receptor Ca2+-signalling. Arch Toxicol. 2021 May;95(5):1703-1722. (2021)

Klima S, Brüll M, Spreng AS, Suciu I, Falt T, Schwamborn JC, Waldmann T, Karreman C, Leist M

➥Read the article

Development of a neural rosette formation assay (RoFA) to identify neurodevelopmental toxicants and to characterize their transcriptome disturbances. Arch Toxicol. 2020 Jan;94(1):151-171. doi: 10.1007/s00204-019-02612-5. Epub 2019 Nov 11. PMID: 31712839. (2020)

Dreser N, Madjar K, Holzer AK, Kapitza M, Scholz C, Kranaster P, Gutbier S, Klima S, Kolb D, Dietz C, Trefzer T, Meisig J, van Thriel C, Henry M, Berthold MR, Blüthgen N, Sachinidis A, Rahnenführer J, Hengstler JG, Waldmann T, and Leist M

➥Read the article

Establishment of a human iPS cell-derived neuronal model cell to study synaptic signaling (2020)

Traub S

➥Read the article

The EU-ToxRisk method documentation, data processing and chemical testing pipeline for the regulatory use of new approach methods. Arch Toxicol. 2020 Jul;94(7):2435-2461. doi: 10.1007/s00204-020-02802-6. Epub 2020 Jul 6. PMID: 32632539; PMCID: PMC7367925. (2020)

Krebs A, van Vugt-Lussenburg BMA, Waldmann T, Albrecht W, Boei J, Ter Braak B, Brajnik M, Braunbeck T, Brecklinghaus T, Busquet F, Dinnyes A, Dokler J, Dolde X, Exner TE, Fisher C, Fluri D, Forsby A, Hengstler JG, Holzer AK, Janstova Z, Jennings P, Kisitu J, Kobolak J, Kumar M, Limonciel A, Lundqvist J, Mihalik B, Moritz W, Pallocca G, Ulloa APC, Pastor M, Rovida C, Sarkans U, Schimming JP, Schmidt BZ, Stöber R, Strassfeld T, van de Water B, Wilmes A, van der Burg B, Verfaillie CM, von Hellfeld R, Vrieling H, Vrijenhoek NG, and Leist M

➥Read the article

Examination of microcystin neurotoxicity using central and peripheral human neurons. ALTEX. 2021;38(1):73-81. doi: 10.14573/altex.2003182. Epub 2020 Jun 23. PMID: 32591837. (2020)

Klima S, Suciu I, Hoelting L, Gutbier S, Waldmann T, Dietrich DR, and Leist M

➥Read the article

Template for the description of cell-based toxicological test methods to allow evaluation and regulatory use of the data. ALTEX. 2019;36(4):682-699. doi: 10.14573/altex.1909271. Erratum in: ALTEX. 2020;37(1):164. PMID: 31658359. (2019)

Krebs A, Waldmann T, Wilks MF, Van Vugt-Lussenburg BMA, Van der Burg B, Terron A, Steger-Hartmann T, Ruegg J, Rovida C, Pedersen E, Pallocca G, Luijten M, Leite SB, Kustermann S, Kamp H, Hoeng J, Hewitt P, Herzler M, Hengstler JG, Heinonen T, Hartung T, Hardy B, Gantner F, Fritsche E, Fant K, Ezendam J, Exner T, Dunkern T, Dietrich DR, Coecke S, Busquet F, Braeuning A, Bondarenko O, Bennekou SH, Beilmann M, and Leist M

➥Read the article

Major changes of cell function and toxicant sensitivity in cultured cells undergoing mild, quasi-natural genetic drift. Arch Toxicol. 2018 Dec;92(12):3487-3503. doi: 10.1007/s00204-018-2326-5. Epub 2018 Oct 8. Erratum in: Arch Toxicol. 2019 Jun;93(6):1771. PMID: 30298209; PMCID: PMC6290691. (2018)

Gutbier S, May P, Berthelot S, Krishna A, Trefzer T, Behbehani M, Efremova L, Delp J, Gstraunthaler G, Waldmann T, Leist M

➥Read the article

Recommendation on test readiness criteria for new approach methods in toxicology: Exemplified for developmental neurotoxicity. ALTEX. 2018;35(3):306-352. doi: 10.14573/altex.1712081. Epub 2018 Feb 23. Erratum in: ALTEX. 2019;36(3):506. PMID: 29485663; PMCID: PMC6545888. (2018)

Bal-Price A, Hogberg HT, Crofton KM, Daneshian M, FitzGerald RE, Fritsche E, Heinonen T, Hougaard Bennekou S, Klima S, Piersma AH, Sachana M, Shafer TJ, Terron A, Monnet-Tschudi F, Viviani B, Waldmann T, Westerink RHS, Wilks MF, Witters H, Zurich MG, and Leist M

➥Read the article

Stage-specific metabolic features of differentiating neurons: Implications for toxicant sensitivity. Toxicol Appl Pharmacol. 2018 Sep 1;354:64-80. doi: 10.1016/j.taap.2017.12.013. Epub 2017 Dec 24. Erratum in: Toxicol Appl Pharmacol. 2019 Jun 1;372:70. PMID: 29278688. (2018)

Delp J, Gutbier S, Cerff M, Zasada C, Niedenführ S, Zhao L, Smirnova L, Hartung T, Borlinghaus H, Schreiber F, Bergemann J, Gätgens J, Beyss M, Azzouzi S, Waldmann T, Kempa S, Nöh K, and Leist M

➥Read the article

Adverse outcome pathways: opportunities, limitations and open questions. Arch Toxicol. 2017 Nov;91(11):3477-3505. doi: 10.1007/s00204-017-2045-3. Epub 2017 Oct 19. PMID: 29051992. (2017)

Leist M, Ghallab A, Graepel R, Marchan R, Hassan R, Bennekou SH, Limonciel A, Vinken M, Schildknecht S, Waldmann T, Danen E, van Ravenzwaay B, Kamp H, Gardner I, Godoy P, Bois FY, Braeuning A, Reif R, Oesch F, Drasdo D, Höhme S, Schwarz M, Hartung T, Braunbeck T, Beltman J, Vrieling H, Sanz F, Forsby A, Gadaleta D, Fisher C, Kelm J, Fluri D, Ecker G, Zdrazil B, Terron A, Jennings P, van der Burg B, Dooley S, Meijer AH, Willighagen E, Martens M, Evelo C, Mombelli E, Taboureau O, Mantovani A, Hardy B, Koch B, Escher S, van Thriel C, Cadenas C, Kroese D, van de Water B, and Hengstler JG

➥Read the article

Combination of multiple neural crest migration assays to identify environmental toxicants from a proof-of-concept chemical library. Arch Toxicol. 2017 Nov;91(11):3613-3632. doi: 10.1007/s00204-017-1977-y. Epub 2017 May 5. PMID: 28477266. (2017)

Nyffeler J, Dolde X, Krebs A, Pinto-Gil K, Pastor M, Behl M, Waldmann T, and Leist M

➥Read the article

Stem Cell-Derived Immature Human Dorsal Root Ganglia Neurons to Identify Peripheral Neurotoxicants. Stem Cells Transl Med. 2016 Apr;5(4):476-87. doi: 10.5966/sctm.2015-0108. Epub 2016 Mar 1. PMID: 26933043; PMCID: PMC4798731. (2016)

Hoelting L, Klima S, Karreman C, Grinberg M, Meisig J, Henry M, Rotshteyn T, Rahnenführer J, Blüthgen N, Sachinidis A, Waldmann T, and Leist M

➥Read the article

Identification of transcriptome signatures and biomarkers specific for potential developmental toxicants inhibiting human neural crest cell migration. Arch Toxicol. 2016 Jan;90(1):159-80. doi: 10.1007/s00204-015-1658-7. Epub 2015 Dec 26. PMID: 26705709; PMCID: PMC4710658. (2016)

Pallocca G, Grinberg M, Henry M, Frickey T, Hengstler JG, Waldmann T, Sachinidis A, Rahnenführer J, and Leist M

➥Read the article

A LUHMES 3D dopaminergic neuronal model for neurotoxicity testing allowing long-term exposure and cellular resilience analysis. Arch Toxicol. 2016 Nov;90(11):2725-2743. doi: 10.1007/s00204-015-1637-z. Epub 2015 Dec 8. PMID: 26647301; PMCID: PMC5065586. (2016)

Smirnova L, Harris G, Delp J, Valadares M, Pamies D, Hogberg HT, Waldmann T, Leist M, and Hartung T

➥Read the article

A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors. Arch Toxicol. 2015 Sep;89(9):1599-618. doi: 10.1007/s00204-015-1573-y. Epub 2015 Aug 14. PMID: 26272509; PMCID: PMC4551554. (2015)

Rempel E, Hoelting L, Waldmann T, Balmer NV, Schildknecht S, Grinberg M, Das Gaspar JA, Shinde V, Stöber R, Marchan R, van Thriel C, Liebing J, Meisig J, Blüthgen N, Sachinidis A, Rahnenführer J, Hengstler JG, and Leist M

➥Read the article

Grouping of histone deacetylase inhibitors and other toxicants disturbing neural crest migration by transcriptional profiling. Neurotoxicology. 2015 Sep;50:56-70. doi: 10.1016/j.neuro.2015.07.008. Epub 2015 Jul 31. PMID: 26238599. (2015)

Dreser N, Zimmer B, Dietz C, Sügis E, Pallocca G, Nyffeler J, Meisig J, Blüthgen N, Berthold MR, Waldmann T, and Leist M

➥Read the article

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation. J Vis Exp. 2015 Jun 17;(100):e52333. doi: 10.3791/52333. PMID: 26132533; PMCID: PMC4544843. (2015)

Shinde V, Klima S, Sureshkumar PS, Meganathan K, Jagtap S, Rempel E, Rahnenführer J, Hengstler JG, Waldmann T, Hescheler J, Leist M, and Sachinidis A

➥Read the article

Toxicogenomics directory of chemically exposed human hepatocytes. Arch Toxicol. 2014 Dec;88(12):2261-87. doi: 10.1007/s00204-014-1400-x. Epub 2014 Nov 16. PMID: 25399406. (2014)

Grinberg M, Stöber RM, Edlund K, Rempel E, Godoy P, Reif R, Widera A, Madjar K, Schmidt-Heck W, Marchan R, Sachinidis A, Spitkovsky D, Hescheler J, Carmo H, Arbo MD, van de Water B, Wink S, Vinken M, Rogiers V, Escher S, Hardy B, Mitic D, Myatt G, Waldmann T, Mardinoglu A, Damm G, Seehofer D, Nüssler A, Weiss TS, Oberemm A, Lampen A, Schaap MM, Luijten M, van Steeg H, Thasler WE, Kleinjans JC, Stierum RH, Leist M, Rahnenführer J, and Hengstler JG

➥Read the article

Profiling of drugs and environmental chemicals for functional impairment of neural crest migration in a novel stem cell-based test battery. Arch Toxicol. 2014 May;88(5):1109-26. doi: 10.1007/s00204-014-1231-9. Epub 2014 Apr 2. PMID: 24691702; PMCID: PMC3996367. (2014)

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From transient transcriptome responses to disturbed neurodevelopment: role of histone acetylation and methylation as epigenetic switch between reversible and irreversible drug effects. Arch Toxicol. 2014 Jul;88(7):1451-68. doi: 10.1007/s00204-014-1279-6. Epub 2014 Jun 17. PMID: 24935251; PMCID: PMC4067541. (2014)

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Cell Based Assay Development Service FAQs

What techniques do you use to measure endpoints?

We use a wide range of techniques to measure endpoints, e.g. FACS, Immunostaining, ELISA, Western blot and qPCR.

Based on which cell line types can you develop assays?

Our scientific experts can develop assays based on basic cell lines, customized cell lines or iPSC-derived cell types. For this, we use our in-house differentiation protocols with cardiomyocytes, hepatocyte-like cells or neurons for your analysis. Alternatively, you can provide us with your protocol so that after the technology transfer, our experts perform the work using this protocol internally at trenzyme.

Is it possible to order both, assay development and subsequent analysis of compounds?

Yes, our assay experts are delighted to develop an assay according to your needs prior to compound analysis.

What does trenzyme’s cell-based assay development service offer?

trenzyme’s cell-based assay services provide customized solutions for drug discovery, biologics characterization and quality control. We offer a wide range of technical endpoints including flow cytometry assays, PCR and ELISA measurements for our in vitro cell-based assays, tailored to your specific research needs. Our expertise ensures reliable and reproducible results, supporting the development of high-quality therapeutics.

With extensive experience in cell-based in vitro assays, we design and optimize cell-based assays for applications such as potency testing, mode-of-action studies, and biomarker analysis. Whether you need cell-based assays for early-stage research or validated assays for regulatory submission, trenzyme delivers high-performance solutions.

Let us help accelerate your drug development with our state-of-the-art cell-based assay development services!

 

Which cell-based assays can you perform?

trenzyme can perform any in vitro cell-based assay as long as your specific endpoint can be measured with our modern and state-of-the art technical equipment. We can perform flow cytometry, (RT)-qPCR, ELISAs, reporter assays (colorimetry, fluorescence, luminicence) and fluorescence microscopy. All these technical endpoints can be combined with your desired in vitro cell-based assays.

Which cells do you use for your cell-based assay service?

In principal, we can use any cell line for our in vitro cell based assays. If you want to outsource your specific cell-based assay we organize the technology transfer from your laboratory to ours including your cell line. Due to our long-lasting experience we can culture any cell line you may need for our cell-based assay service.

Which cells / cell lines have you already used for your cell-based assay service?

In the past we used many different cell lines for our in vitro cell based assays. There is a wide range from simple cell lines such as HEK290 over more complex cellular systems (e.g. immortalized myoblasts differentiated to mature skeletal muscle cells) to iPSC-derived cell types such as human microglia that can be used for neuroinflammation cell-based assays.

See General FAQs
Team Members of trenzyme's Advanced Cell Services Department. It is a collage in which trenzyme's stem cell experts are depicted. One scientist is wearing glasses and has a pipette in his hand. He is also wearing blue gloves. The scientist in the centre is sitting in front of a microscope. The scientist on the far right is wearing a white lab coat. They all look very friendly and competent and are smiling at the camera. They are all wearing white lab coats.

Our Advanced Cell System Experts

Department of Advanced Cell Systems

We would be happy to provide you with support on your cell culture research project. Contact us and let us know your questions and requests to our cell culture services. Our scientific experts will reply shortly.

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