SARS-CoV-2 (COVID-19) 3CL-Mpro Protein, Tag-free

The cysteine protease 3CL-Mpro of SARS-CoV-2 (COVID-19) cleaves the transcribed viral polyprotein at two self cleavage sites. Here, the polyprotein is cleaved into several functional proteins by the 3CL-M protease.

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  • 100µg – 440€
  • more on request – bulk and gram quantities available

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Overview

Product Name SARS-CoV-2 (COVID-19) 3CL-Mpro Protein, tag-free
Catalog No. P2020-019
RefSeq Links NC_045512.2; MN908947.3; YP_009724390.1; QHD43416.1; GeneID: 43740568
Synonyms 3CL Mpro; 3CL Pro; 3CL protease; 3C-like main protease; SARS-CoV-2; coronavirus; 2019-nCoV; COVID-2019; COVID-19

Sequence Information

Species SARS-CoV-2; Wuhan seafood market pneumonia virus
Tags tag-free
Sequence without tags (AA 1-306) SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIR
KSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNG
SPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGN
FYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYE
PLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQC
SGVTFQ

Product Information

Expression Host E. coli
Formulation PBS, pH 7.4; contains Glycerol as protectant
Format Liquid, stored and shipped at -80°C
Purity > 90% as determined by SDS-PAGE

Background Information

 

The new coronavirus SARS-CoV-2 expresses two proteases, the papain-like protease (PLpro) and 3C-like protease (3CLpro). Both belong to the group of cysteine proteases, as they have a cysteine residue at their catalytic site. Their main function is the processing of the viral polyprotein, that contains two cleavage sites to build up the viral replicase complex. Additionally, PLpro has the ability of removing ISG15 and ubiquitin from viral proteins expressed in the cell, this enables evasion from the innate immune response by the host. This represents an interesting target for drug development. This is because it not only would inhibit viral replication, but would also prevent the massive immunological response that results from the over-activation of the host’s immune system. Such an over-activation can lead to damage of the uninfected cells and thus to a worsening of the patient’s condition.

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture

SDS-PAGE of 3CL-Mpro Protein, tag-free Histogram (of marked lane in gel picture) of 3CL-Mpro Protein, tag-free

Inhibition of Protease 3CL-Mpro by GC376 (2% DMSO)

Graph showing inhibition of Protease 3CL-Mro by GC376

References and further readings

Further recombinant SARS-CoV-2 proteins

Learn more about our other recombinant SARS-CoV-2 proteins.
Reinhold Horlacher, PhD

Reinhold Horlacher, PhD

Managing Director & CSO

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