Feasibility Check & Project Consultion
The feasibility study involves a close dialogue between the customer and our cell culture experts. The customer's requirements are discussed and finally our cell culture experts will suggest you one or more reasonable medium compositions.
Once the customized quotation has been prepared by our team and accepted by the customer, the project can begin.
QC of Incoming Cell Line
During this step of the project we will confirm the culture conditions of the external cell line including mycoplasma test. This step is not necessary when a trenzyme in-house cell line will be used for the project.
- Thawing and cultivation of cells
- Thaw-viability assay, microscopic evaluation and mycoplasma test
- Verification of culture conditions
- Cryopreservation of a small cell bank
Cell Line Adaption
Depending on the cell line that should be adapted it can be beneficial to perform a stepwise adaption or to perform a so-called “do-or-die”-approach.
During adaption the growth of your cell line will be monitored and documented. You will receive full documentation and medium composition in a comprehensive data sheet or optionally a final report. Viability and growth rates will be monitored and documented over several passages for both approaches
- Step-Wise Adaption: The old medium will be replaced slowly in several steps (e.g. 25%, 50%, 75%, 100%)
- „Do-or-Die“-Approach: The old medium will be replaced in one step at once
Cell Line Expansion & Cryopreservation
The adapted cells will be maintained in culture for a few passages in order to validate successful adaption to the new medium. After validation we will expand the cells and cryopreserve them according to your needs (freezing media, number of cells / vial, number of vials).
- Expansion of adapted cell line
- Cryopreservation of a cell bank
Quality Control of the Adapted Cell Line
We thaw your newly adapted cryopreserved cell line to scrutinise viability of the cell line. In order to verify the culture conditions we will passage the cells for another three times.
Standard Quality Control:
- Thaw-Viability Assay
- Mycoplasma Test for your security
Further Optional Analysis Methods According to Your Needs:
- Flow Cytometry ( i.e. expression of specific proteins/genes)
- Immunflourescence
- PCR
- Western Blot
- ELISA
- RT-qPCR
Shipment - Fast Delivery
- Worldwide Shipping
We offer global shipping while adhering to embargo regulations, ensuring reliable delivery to your location. - Fast Delivery Times
Our logistics team works with trusted partners to guarantee swift delivery without compromising product quality. - Reliable Logistics Network
We collaborate with renowned delivery providers who operate efficient worldwide logistics systems. - Parcel Tracking for Transparency
You’ll receive a tracking code upon dispatch, allowing continuous monitoring of your shipment's progress.
Deliverables
Together with your cryopreserved cells you will obtain comprehensive documentation including
- Data sheet with exact culture conditions and media compositions
- Growth rate documentation
- Optional: a full report about the whole adaption process
Timeline
- The timeline very much depends on the cell line, the doubling rate of the cells and on the type of medium (low or high nutrient content) to which the cells shall be adapted. Therefore timeline ranges from 6 to 12 weeks.