Characterization of human induced
Pluripotent Stem Cells
The field of human induced pluripotent stem cell (hiPSC) technology is a promising tool for drug discovery and regenerative medicine.
Since the first generation of hiPSCs, numerous reprogramming techniques have been established. However, the in-vitro process of reprogramming is still not fully understood. Often, the cellular stress, induced by the reprogramming process causes genetic and epigenetic alterations that can change the cellular phenotype and affect the integrity of experiments using these cell lines.
It is therefore crucial to establish reliable molecular and functional assays to assess pluripotency and to exclude genetic aberrations due to the reprogramming process.
trenzyme’s routinely performed assays to validate your hiPSC lines include the characterization of cells for their expression of pluripotency markers via immunocytochemistry (ICC) and qPCR, molecular karyotyping, as well as the evaluation of their differentiation potential.
What clients say about us
iPSC Characterization
& Banking Service
If you work in the field of stem cell research and you are reprogramming hiPSC lines or start experiments using a unknown hiPSC line, our scientific stem cell experts support you with fully characterized and ready-to-use hiPSC master and working stocks. Accordingly, trenzyme serves as your time- and cost-saving extended workbench.
Our expert service includes: